沉默MEF2C基因对髓母细胞瘤Daoy细胞形态结构的影响

Influence of silencing MEF2C genes on morphological structure of medulloblastoma Daoy cell

目的探讨抑制人MEF2C基因后对髓母细胞瘤Daoy细胞形态结构的影响作用。方法实验分为两组:对照组和干扰组。对照组Daoy细胞培养感染阴性慢病毒液,干扰组Daoy细胞培养感染MEF2C-RNAi慢病毒液。光镜下观察培养细胞的大体形态,然后利用透射电镜观察细胞的超微结构变化情况。同时将两组细胞分别接种至裸鼠背部皮下,观察裸鼠成瘤情况以及肿瘤组织的光镜下结构和电镜下超微结构变化情况。结果干扰组Daoy细胞生长较对照组细胞生长缓慢,种植裸鼠后成瘤重量较对照组轻;光镜下显示对照组瘤组织细胞较干扰组瘤组织细胞排列密集;电镜下显示干扰组瘤组织细胞排列疏松,核固缩,凋亡小体形成。结论沉默MEF2C基因可以引起异染色质减少、凋亡小体形成,影响细胞的形态结构,抑制肿瘤细胞生长,为其肿瘤生物治疗提供了实验依据。

Objective To explore the effect of silencing MEF2C gene on the morphological structure of medulloblastoma Daoy cell.Methods The experiment was divided into two groups：control group and interference group.Daoy cells of control group were infected with negative LV liquid,and Daoy cells of interference group were infected with MEF2C-RNAi-LV liquid.Light-microscopy （LM） was used to observe general form of cells,and transmission electron microscopy （TEM） was used to observe ultrastructure changes of cells.Meanwhile,the two groups of cells were subcutaneously inoculated to the dorsum of nude mice to forn transplantation tumors,respectively,and the morphological structures of tumor tissues were observed by LM and TEM.Results Daoy cells of interference group grew slower than cells of control group,the weights of transplantation tumors in interference group were lighter than those in control one.The tumor tissue cells of control group had more intensive array than those of the interference group;Tumor tissue cells of interference group showed array loose,nucleus pycnosis,apoptosis body formation under TEM.Conclusions Silencing MEF2C gene could cause heterochromatin reduce,apoptosis body formation,and influence cell morphology,inhibit tumor cell growth.This provides experimental basis for tumor biological therapy.