HPV16/18在宫颈癌组织中的表达及其临床意义

Expression and clinical significance of HPV 16/18 in cervical carcinoma

目的探讨人乳头瘤病毒(HPV)16/18在宫颈癌组织中的表达及其临床意义。方法采用PCR扩增+膜杂交法检测宫颈癌组织(A组,71例)、宫颈上皮内瘤变(CIN,B组,69例)和正常宫颈组织(C组,50例)HPV16/18的表达。结果A、B组HPV16/18阳性率分别为73.24%和24.24%,均高于C组的12.00%(P<0.05),A组高于B组(P<0.05)。A组CINⅢ级患者HPV16/18阳性率高于Ⅰ-Ⅱ级(P<0.05)。结论宫颈癌组织中HPV16/18阳性表达率高于CIN及正常宫颈组织;对宫颈病变检查者行常规的HPV16/18检测和对怀疑为CIN患者进行病理活检有助于宫颈恶性病变的早期诊断和治疗。

Objective To investigate the expression and clinical significance of human papillomavirus（HPV） 16/18 in cervical carcinoma. Methods With PCR amplification and membrane hybridization assay, the expression of HPV 16/18 was detected in cervical cancer（group A, 71 cases）, cervical intraepithelial neoplasia （CIN, group B, 69 cases）, and normal cervical tissues （group C, 50 cases）. Results The positive expression rates of HPV 16/18 in groups of A and B were 73.24% and 24. 24%, respectively, which were significantly higher than 12. 00% of group C （P〈0. 05）. The positive expression rates of HPV 16/18 were higher in group A than those in group B（P〈0. 05）. The positive expression rates of HPV 16/18 in cervical cancer were higher in CIN Ⅲ grade than those in CIN Ⅰ-Ⅱ grade（P〈0. 05）. Conclusion The positive expression rates of HPV 16/18 are significantly higher in cervical carcinoma than those in CIN and normal cervical tissues. A routine examination of HPV16/18 in cervical lesions and pathological biopsy of suspected CIN are helpful in early diagnosis and treatment of malignant cervical diseases.