摘要
目的对比3种人主要组织相容性复合物I类相关链A位点（MICA）特异性抗体检测试剂结果的一致性，预测其临床推广应用的价值。方法18家实验室参加16届国际组织相容性和免疫协作组，均采用免疫磁珠流式细胞仪液相芯片技术检测4批16份血清样本的MICA抗体的特异性，3种试剂标记为Kit-A、Kit-B、Kit-C。结果在16份血清样本中，804为阴性，15份样本结果阳性，其中S10样本各实验室均上报为阳性。依据MICA抗原公共表位分G1（MICA 01、02、07、12、17、18）组和G2（MICA04、06、08／27、09、19）组，5例样本用Kit—A和Kit-B均能判别MICA-G1组特异性抗体，Kit-C存在MICA08／27和MICA19的假阳性结果。5例样本用Kit—A和Kit-B均能判别MICA-G2组特异性抗体。其余5例样本用Kit-A、Kit—B和Kit-C均无法判别MICA特异性抗体一致的结果。从3种试剂均能判别10种MICA特异性抗体结果分析，MICA08／27（86．67％，13／15）抗体结果一致性最高，MICA19（40％，6／15）一致性最低。使用Kit—B试剂的实验室间有13例MICA特异性抗体样本结果符合率大于80％。结论协作组实验室对于判断MICA抗体阴性或阳性没有差异，而对MICA抗体特异性分析存在不一致性，因此，建议选择MICA特异性抗体类型较宽检测试剂或最好选择两种以上的试剂进行检测。
Objective To research the consistency of testing results with three different anti- major histocompatibility complex class I -related chain A（MICA） specific antibody reagents in order to evaluate their clinical application＇s value. Method An collaborative study of 18 laboratories was undertaken at the 16th International HLA and Immunogenetics Workshop. Total of 16 sera（4 batchs） were tested for anti-MICA antibodies by Luminex method with three different reagents （Kit-A, -B and -C）. Result Anti-MICA antibodies were found in 15 sera, except one sera（no. S04） ; No. S]0 sera showed positive results in all the laboratories. The anti-MICA antibodies were divided into MICA-G1 group （MICA01, 02, 07, 12, 17 and 18） and MICA-G2 group （MICA 04, 06, 08/27, 09 and 19）. MICA-G1 group specific antibodies were detected in 5 sera with Kit-A and -B reagent but there were false-positive results of anti-MICA08/27 and MICA19 antibodies in this 5 sera with KivC. MICA-G2 group specific antibodies can be detected in other 5 sera with Kit-A and -B, But the MICA specific antibodies testing gave different results with Kit-A, -B and -C in all the last 5 sera samples. Testing of MICA08/27 showed highest consistency results （86. 67~, 13/15） with Ki^A, -B and C; and testing of MICA19 showed lowest consistency results （40%, 6/15） with this 3 reagents. There were 80O//oo consistency results of anti-MICA specific antibodies in 13 sera with Kit 13. Conclusion There are the same effect to judgment positive or negative result for anti-MICA antibodies with 3 different reagents,but the results of anti-MICA specific antibodies are not the same. Therefore, it＇s better to use two or more reagents to test anti-MICA specific antibodies, or choose reagent with wide detection range.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2014年第5期290-294,共5页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金（81273266,30872530）
卫生部重大科研基金（LW201002）
江苏省医学创新团队与领军人才（LJ201138）
江苏省医学重点学科（XK201151）
江苏省科技支撑计划项目（BE2010651）
江苏省临床医学中心（ZX201102）
苏州市科技基础设施建设计划项目（SZS201001,szS0702）
