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Piezos离子通道在大鼠牙周组织中表达的实验研究 被引量：8

Piezos' expression in periodontal tissues of rats
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摘要 目的:通过观察机械敏感离子通道Piezos蛋白在牙周组织中的表达情况,探讨其在牙周组织机械感受中的作用。方法:使用间接免疫荧光技术,在蛋白水平观察机械敏感离子通道Piezos蛋白在大鼠牙周组织的表达特点及定位。结果:Piezo1和Piezo2在牙周膜成纤维细胞中均呈阳性表达,其中Piezo2的表达量明显低于Piezo1(P<0.05);Piezos两种蛋白主要表达于牙周膜成纤维细胞,但在骨细胞中未见表达;Piezos在细胞中的表达部位主要在胞膜上,部分在胞浆里。结论:牙周组织,特别是牙周膜中有丰富的Piezos离子通道的表达,提示Piezos可能参与了牙周膜的感觉传导。 AIM： To observe Piezo protein expression in periodontal tissues. METHODS：Piezo expression in periodontal tissues of rats was detected by indirect immunofluorescence technique. RESULTS ： Positive staining of Piezol and Piezo2 was observed in periodontal fibroblasts of rats while the expression of Piezo2 was obviously lower than that of Piezol （P 〈 0.05）. Piezos were mainly expressed in periodontal ligament fibroblasts, but no positive staining was found in osteocytes. Piezos＇ expression in the plasma membrane of periodontal ligament fibroblasts was higher than that in the cytoplasm. CONCLUSION： There is a rich expression of Piezos ion channel in periodontal ligament. Piezos play a role in perceptive function of periodontal ligament.
作者 康婷 黄世友 李鹏 史婷 余擎 段银钟
机构地区 第四军医大学口腔医院正畸科军事口腔医学国家重点实验室 解放军第 成都军区机关医院 河南护理职业学院
出处 《牙体牙髓牙周病学杂志》 CAS 北大核心 2014年第5期269-273,共5页 Chinese Journal of Conservative Dentistry
基金 国家自然科学基金(31271048 81170948)
关键词 牙周组织 Piezo 离子通道 机械敏感 periodontal tissue piezo ion channel mechanosensitive
分类号 R780.2 [医药卫生—口腔医学]
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参考文献20

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二级参考文献12

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牙体牙髓牙周病学杂志
2014年 第5期

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