三氯乙烯致敏小鼠肾损伤中补体C3a和C5a水平

Levels of complement components C3a and C5a in renal injury among trichloroethylene-sensitized BALB/c mice

目的 检测三氯乙烯（TCE）致敏小鼠不同时期肾脏中补体C3a和C5a水平,探讨在TCE致敏引起的肾损伤中补体的作用.方法 小鼠经皮内注射和腹部涂抹TCE 2次致敏后激发.在末次激发后的24 h、48 h、72 h和7d4个时间点采血、取肾脏,用全自动生化分析仪检测血清中尿索氮（BUN）和肌酐（Cr）水平,免疫组织化学法检测肾脏中C3a和C5a沉积水平.结果 TCE处理组的小鼠致敏率达到42.0％.与溶剂对照组比较,致敏48 h和72 h组小鼠肾脏系数、血清BUN和Cr水平均明显升高,差异有统计学意义（P＜0.05）.与同时点末致敏组比较,48 h和72 h致敏组小鼠肾脏系数明显增大,差异有统计学意义（P＜0.05）.与溶剂对照组比较,7d致敏组肾脏系数、血清BUN、Cr水平差异无统计学意义（P＞0.05）.与溶剂对照组比较,致敏48 h组小鼠肾脏补体C3a（3.80±0.84）、C5a （4.00± 1.00）水平明显升高,差异有统计学意义（P＜0.05）;致敏72 h组小鼠肾脏中补体C3a（4.40±1.14）、C5a（4.40±1.14）水平明显高于溶剂对照组,差异有统计学意义（P＜0.05）.7d致敏组小鼠肾脏中补体C3a（1.80±0.45）、C5a （2.00±0.71）水平与溶剂对照组比较,差异无统计学意义（P＞0.05）.结论 TCE致敏可引起小鼠肾功能损伤,补体C3a和C5a在致敏小鼠肾脏中的水平升高,提示补体C3a和C5a可能参与TCE致敏引起的肾脏损伤.

Objective To determine the levels of complement components C3a and C5a in the kidneys of trichloroethylene （TCE）-sensitized BALB/c mice,and to investigate the role of complement components in TCE-induced renal injury among BALB/c mice.Methods Sixty-two female BALB/c mice were randomly divided into blank control group,vehicle control group,and TCE sensitization group.The mice in TCE sensitization group were sensitized by one intracutaneous injection and one abdominal smear of TCE.At 24 h,48 h,72 h,and 7 d after the second sensitization,mice were sacrificed,and the blood and kidneys were collected.An automatic biochemical analyzer was used in the determination of serum blood urea nitrogen （BUN） and creatinine （Cr）.The levels of C3a and C5a in the kidneys were determined by immunohistochemistry.Results The sensitization rate of TCE sensitization group was 42.0％.Kidney coefficient and serum levels of BUN and Cr were significantly increased in the TCE sensitization group as compared with the vehicle control group at 48 h and 72 h after sensitization （P＜0.05）.The kidney coefficients of the TCE sensitization group at 48 h and 72 h were significantly higher than those of the control groups （P＜0.05）.In comparison with the vehicle control group,however,no significant change was found in kidney coefficient,serum BUN,or serum Cr at 7 d after TCE sensitization （P＞0.05）.Levels of C3a and C5a at 48 h （3.80±0.84 and 4.00±1.00,respectively） and 72 h （4.40±1.14 and 4.40±1.14,respectively） after sensitization were all significantly higher than those of the vehicle control group （P＜0.05）,but no significant difference was found in level ofC3a （1.80±0.45） or C5a （2.00± 0.71） at 7 d （P＞0.05）.Conclusion TCE sensitization can induce renal injury in mice.Levels of complement components C3a and C5a are elevated in the kidneys of sensitized mice,indicating that C3a and C5a may be involved in the renal injury induced by TCE sensitization.