大蒜油对苯致小鼠血液系统损伤的预防作用

Preventive effects of garlic oil against the benzene-induced hematotoxicity in mice

目的 研究大蒜油对苯中毒致小鼠血液系统损伤的预防作用.方法 SPF级雄性昆明小鼠100只,随机分为对照组、模型组、低、中、高剂量大蒜油干预组,每组20只.各大蒜油干预组小鼠每日分别经口给予20、40和80 mg/kg的大蒜油,其余2组小鼠给予等体积的玉米油.2h后,除对照组小鼠外,其余各组动物均经口给予体积分数为20％的苯（1：4溶解于玉米油中）10 ml/kg,连续21d.第22天,各组动物眼球取血,全自动血球计数仪测定红细胞（RBC）、白细胞（WBC）、血小板（PLT）及血红蛋白（Hb）的含量;取脾脏,称重并计算脏器系数.结果 与对照组比较,苯中毒模型组WBC总数减少了40％,RBC和PLT分别减少了18％和28％,Hb含量下降了23.6％,差异均有统计学意义（P＜0.01）.与苯中毒模型组比较,高剂量大蒜油组WBC增加了95％,其中淋巴细胞和单核细胞数量分别增加了142％和100％,高剂量大蒜油组PLT升高了66％,差异有统计学意义（P＜0.01）.低剂量大蒜油组RBC和Hb含量分别增加了15％和16％,差异有统计学意义（P＜0.05）.与苯中毒模型组比较,高剂量大蒜油组小鼠脾重和脾脏系数分别增加366.7％和75.0％,差异有统计学意义（P＜0.05）.结论 大蒜油可抑制苯中毒引起的小鼠血液系统的损伤,其作用机制可能与刺激脾脏造血有关.

Objective To investigate the preventive effects of garlic oil （GO） on benzene-induced hematotoxicity in mice.Methods Specific pathogen-free male Kunming mice were randomly divided into 5 groups,i.e.,control group,model group,and low-,middle-,and high-dose GO groups （n=20 in each group）.Mice in GO groups were orally given GO at 20,40,or 80 mg/kg BW,while mice in the other two groups received an equal volume of corn oil.Two hours later,mice in model group and GO groups were orally given benzene （20％,v/v,dissolved in corn oil,10 ml/kg BW） for 21 days consecutively.On the 22nd day,blood was collected from the orbital sinus,to determine the counts of red blood cells （RBC）,white blood cells （WBC）,and platelets （PLT） and hemoglobin level using an automatic blood cell counter.The mice were sacrificed thereafter.The spleen was excised and weighed for calculation of the spleen index （spleen weight/body weight× 100％）.Results The counts of WBC,RBC,and PLT and Hb level in the model group were reduced by 40％,18％,28％,and 23.6％,respectively,as compared with those in the control group （P＜0.01）.Compared with those in the model group,WBC and PLT counts in the high-dose GO group increased by 95％ and 66％,respectively （P＜0.01）,wherein lymphocytes and monocytes increased by 142％ and 100％,respectively （P＜0.01）; the RBC count and Hb level in the low-dose GO group increased by 15％ and 16％,respectively （P＜0.05）.GO significantly suppressed benzeneinduced decreases in spleen weight and spleen index.Conclusion GO is capable of suppressing benzeneinduced hematotoxicity in mice.One possible mechanism may be promotion of hematopoiesis in the spleen.