甲醛灌胃染毒对小鼠肝脏超氧化物歧化酶和丙二醛的影响

Effects of intragastric administration of formaldehyde on superoxide dismutase activities and malondialdchyde contents in liver of mice

目的 研究甲醛灌胃染毒对小鼠肝脏超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量的影响.方法 ICR小鼠30只,雌雄各半,随机分为3组,每组10只,雌雄各半,染毒剂量为：0、5、20 mg/kg体重,用灌胃的方法给予甲醛,每天1次,连续5d,对照组给予蒸馏水.取肝脏,用试剂盒测定各剂量组SOD活力和MDA含量.结果 与对照组比较,20 mg/kg染毒组小鼠肝脏中SOD活力明显降低,MDA的含量明显升高,差异有统计学意义（P＜0.05）,与对照组比较,20 mg/kg染毒组小鼠肝脏脏器系数明显升高,差异有统计学意义（P＜0.05）.结论 甲醛灌胃染毒对小鼠肝脏的脂质过氧化反应的平衡状态具有破坏作用,使其抗氧化能力明显降低.

Objective To study the effects of intragastric administration of formaldehyde on lipid peroxidation in mice.Methods Thirty ICR mice were randomly divided into 3 groups：one control group and two experimental groups.The mice were given formaldehyde （the dose is 0,5 and 20 mg/kg body weight respectively） through intragastric administration once a day for 5 days,and then they were killed.The activities of SOD and the contents of MDA in liver were measured.Results The activities of SOD in the 20 mg/kg body weight group were significantly lower than the control group （P＜0.05）,and the contents of MDA in the 20 mg/kg body weight group were significantly higher than the control group （P＜0.05）,and the liver organ coefficient in the 20mg/kg body weight group is higher than the control group （P＜0.05）.Conclusion A certain dose of formaldehyde can destroy the balance of lipid peroxidation in mice,the ability of antioxidation is reduced obviously,and the liver become compensatory hypertrophy.