膝关节骨性关节炎患者尿液中C2C水平检测

Detection of urine C2C level in patients with knee osteoarthritis

目的观察并探讨尿液中Ⅱ型胶原羧基末端3/4片段(C2C)水平与膝关节骨性关节炎(KOA)患者病变程度的关系。方法收集90例膝关节骨性关节炎患者及20例非膝关节骨性关节炎患者清晨尿液标本5 mL及膝关节正侧位影像学资料,尿液标本置-80℃冰箱保存,影像学资料根据Kellgren-Lawrence放射学分级标准对膝关节骨性关节炎患者的影像学表现进行分级,实验分为五组,对照组,I级组,II级组,III级组及IV级组。各组间年龄及性别构成比较没有显著性差异,无统计学意义。尿液标本收齐后采用酶联免疫吸附试验(ELISA)检测尿液中C2C水平。结果与对照组(261.235±39.944 pg/mL)相比,膝关节骨性关节炎组C2C水平(218.341±22.270 pg/mL)明显升高,差异有统计学意义(P<0.01)。各组C2C水平比较:IV组(311.872±18.759 pg/mL)>III组(273.753±18.595 pg/mL)>II组(256.251±23.379 pg/mL)>I组(219.009±13.431pg/mL)或正常组(218.341±22.270 pg/mL)(P=0.000)。I级膝关节骨性关节炎组患者尿液C2C与对照组水平相差无统计学意义(P>0.05)。结论骨性关节炎患者C2C水平较正常对照组高,C2C升高水平与膝关节骨性关节炎病变程度平行,且二者呈高度正相关。

Objective To observe the relationship between the urine C2C level and the severity of knee osteoarthritis （KOA）. Methods Ninety patients with KOA were selected as KOA group , and 20 healthy volunteers were selected as control group .Five ml morning urine of each subject was collected and stored at -80℃.The anterior-posterior and lateral position of X-ray detection of knee joint was performed .The X-ray images were classified according to the Kellgren-Lawrence radiographic grading criteria .All the subjects were divided into 5 groups：control group, group Ⅰ, group Ⅱ, group Ⅲ, and group Ⅳ, with no significant difference of age and sex.The urine C2C level was detected using enzyme-linked immunosorbent assay （ELISA）.Results The urine C2C level in KOA group （218.341 ±22.270 pg/mL） was significantly higher than that in control group （261.235 ±39.944 pg/mL） （ P〈0.01）.The urine C2C level in group Ⅳ, group Ⅲ, group Ⅱ, group Ⅰ, and control group was 311.872 ±18.759 pg/mL, 273.753 ±18.595 pg/mL, 256.251 ±23.379 pg/mL, 219.009 ±13.431 pg/mL, and 218.341 ±22.270 pg/mL, respectively, and the difference was significant （P=0.000）.No significant difference of the urine C2C level between group Ⅰ and control group was observed （P＆gt;0.05）.Conclusion The urine C2C level in patients with KOA was higher than that in normal subjects .And the elevation of urine C2C level is parallel to the severity of KOA , showing a high-positive correlation.