Cdc25a在跨种属肝癌组织中的表达及其意义

Expression of Cdc25a in cross-species hepatocellular carcinoma tissues and its significance

目的:研究细胞周期通路中的细胞分裂周期蛋白25a(Cdc25a)在人、大鼠和树鼩不同种属的肝癌、癌旁及正常肝组织中的表达和意义,进一步验证跨种属筛选肝癌关键基因研究策略的可行性。方法:采用实时荧光定量PCR和Western blotting技术检测人、大鼠和树鼩的肝癌及其相应癌旁组织以及正常肝组织中Cdc25a mRNA和蛋白表达水平。结果:Cdc25a mRNA表达水平在人、大鼠和树鼩的肝癌组织中均高于正常肝组织(均P<0.05);在人和大鼠肝癌组织中的表达高于其对应的癌旁组织(P<0.05);其余各组织间mRNA表达水平的差别无统计学意义(均P>0.05)。Cdc25a mRNA在人肝癌组织中的表达水平与病人的临床分期、门脉癌栓及肝外转移明显相关,而与术后复发、肿瘤直径、肿瘤个数、血清甲胎蛋白水平和肿瘤分化无明显关系。Western blotting检测结果显示Cdc25a在人、大鼠和树鼩肝癌组织中的蛋白表达水平均较癌旁和正常肝组织显著上调。结论:Cdc25a有可能是影响肝癌发生和发展的重要分子之一,跨种属筛选肿瘤关键基因策略可能有助于发现肝癌关键基因。

AIM： To study the expression of cell division cycle 25a protein （Cdc25a） in hepatocellular carci-noma （HCC） tissues of different species, including human, rat and tree shrew, and to verify the feasibility of the strategy of cross-species, oncogenomics screening. METHODS： Real-time fluorescence quantitative PCR and Western blotting were applied to detect the expression of Cdc25a at mRNA and protein levels in the HCC tissues, corresponding HCC-adjacent liver tissues and normal liver tissues collected from humans, rats and tree shrews. RESULTS： The mRNA expression of Cdc25a in the HCC tissues of humans, rats and tree shrews was higher than that in normal liver tissues （P 〈 0.05 ）. The mRNA levels of Cde25a in the HCC tissues of humans and rats were higher than those in the corresponding HCC-adjacent liver tissues （P 〈 0.05 ）. The mRNA expression of Cdc25a in the HCC tissues was significantly correlated with the portal vein tumor thrombus, the extrahepatic metastasis and the clinical stage, but was not correlated with the recurrence of tumor, the diameter of tumor, the number of tumor, the level of serum alpha-fetoprotein and the differentiation of tumor. The protein levels of Cdc25a in the HCC tissues of humans, rats and tree shrews were higher than those in the correspond-ing HCC-adjacent liver tissues and the normal liver tissues. CONCLUSION： Cdc25a may be a particularly crucial molecule for hepatocarcinogenesis. The cross-species oncogenomics screening may represent a feasible and convenient way for identifying key molecules of human HCC.