水通道蛋白4在大鼠淋巴性脑水肿中的作用

Effect of aquaporin 4 on lymphatic brain edema in rats

目的:研究水通道蛋白4(AQP4)在淋巴性脑水肿(LBE)形成和消散过程中的作用。方法:将大鼠随机分为假手术组和LBE组。采用结扎双侧颈部淋巴管并摘除淋巴结的方法,制作LBE模型。干湿重法检测大鼠大脑皮质含水量,应用免疫荧光组织化学方法及免疫印迹检测在颈淋巴引流阻滞后不同时点AQP4表达的变化情况,并对AQP4表达与脑含水量作相关分析。结果:与假手术组相比,LBE组大鼠脑含水量、AQP4免疫荧光表达阳性细胞数、强度及AQP4蛋白表达在术后3 d均见升高(P<0.05),7 d升至高峰(P<0.01),后逐渐降低,且AQP4蛋白表达与脑含水量呈正相关关系(r=0.8024,P<0.05)。结论:AQP4在大鼠LBE的形成和消散过程中起到了重要的作用。

AIM ： To investigate the role of aquaporin 4 （ AQP4 ） in the formation and elimination of lymphatic brain edema （LBE）. METHODS ： Sprague-Dawley rats were randomly divided into sham group and LBE group. LBE was induced by ligating bilateral cervical lymphatic tubes and removing the corresponding lymphatic nodes. The water contents in the cerebral cortex were measured by wet-dry weight method. The expression of AQP4 in the cerebral cortex was detected 1 d, 3 d, 7 d, 11 d and 15 d after a cervical lymphatic： blockade or a sham operation by immunohistofluorescence staining and Western blotting. Furthermore, correlation analysis was made between the protein expression of AQP4 and the hrain water content. RESULTS ： Compared with sham group, cerebral water content, and the protein expression of AQP4 in the LBE rats increased significantly 3 d after operation, peaked at 7 d, followed by gradually stepping down. Furthermore, the expression of AQP4 was positively correlated with brain water content （ r = 0. 8024, P 〈 0.05 ）. CONCLUSION ： AQP4 may play an important role in the formation and elimination of LBE.