气相色谱-三重四极杆串联质谱稳定同位素稀释法测定被动吸烟儿童尿液中的可丁宁

Determination of urinarycotinine of children exposed to passive smokingbystable isotope dilution gas chromatographytriple quadrupole mass spectrometry

建立了被动吸烟儿童尿液中可丁宁的气相色谱-三重四极杆串联质谱（ GC-MS / MS）稳定同位素稀释测定方法。尿液经过三氯甲烷提取、净化，采用气相色谱-三重四极杆串联质谱多反应监测（ MRM）模式测定，以可丁宁-d3稳定同位素为内标，定量测定和确证被动吸烟儿童尿液中的可丁宁；在0.1~10μg / L 可丁宁质量浓度范围内方法的线性关系良好，相关系数 r﹥0.998；空白尿液中添加可丁宁0.1、1.0和10μg / L，回收率为79.2％~112.8％，相对标准偏差在2.1％~5.8％之间；方法定量限达到0.1μg / L。该方法准确、灵敏、快速，适用于家庭被动吸烟儿童尿液中可丁宁的测定。

An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry（ GC-MS / MS）. The samples were extracted and purified with chloroform. The extracts were determined by GC-MS / MS in multiple reaction monitoring （MRM）mode. The cotinine-d 3 as an isotope internal standard was applied to quantify and con-firm the urinary cotinine of children exposed to passive smoking. The method had a good linear-ity from 0. 1 μg / L to 10 μg / L with the correlation coefficient（r）﹥0. 998. The recoveries of the cotinine in blank urine were from 79. 2% to 112. 8% at spiked levels of 0. 1,1. 0 and 10 μg / L, with relative standard deviations（RSDs）from 2. 1% to 5. 8% . The limit of quantification（LOQ） of the method was 0. 1 μg / L. The developed method is accurate,sensitive,rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.