角膜不同保存方法与术后免疫排斥反应相关性的比较研究

Comparative study on the correlation of penetrating keratoplasty rejection with different cornea preservation methods

目的:探讨角膜不同保存方法与术后免疫排斥反应的关系。方法:随机用Wistar大鼠30只作为供体,SD大鼠60只作为受体。建立角膜移植排斥反应动物模型。将SD大鼠随机分3组,各组分别使用不同保存方法的Wistar鼠眼为供体角膜。观察3组角膜移植术后排斥反应指数(RI)、植片存活时间(MST)和植片的病理变化。结果:MST湿房保存组(Ⅰ组)为10.4±1.70d,中期保存液保存组(Ⅱ组)为12.9±1.81d,深低温保存组(Ⅲ组)为16.1±2.57d。Ⅲ组MST明显延长,与其他两组比较均有显著性(P<0.01)。角膜移植术后10d时HE染色显示Ⅲ组炎性反应较Ⅰ组、Ⅱ组明显减轻。结论:深低温保存的大鼠角膜移植术后排斥反应发生率降低,发生时间延迟。

AIM：To understand the relation between the penetrating keratoplasty rejection and the methods of cornea preservation. METHODS： The 30 Wistar rats as donator and 60 SD rats as receptor were used to establish the animal models of penetrating keratoplasty rejection. And 60 SD rats were randomly divided into 3 groups. Donor cornea of Wistar rats preserved in different methods were used separately in 3 groups. The penetrating keratoplasty rejection index（RI）, means survival time（MST）of corneal grafts and pathological changes in post-operation were analyzed. RESULTS： The MST was（10.4±1.70）d in moist-chamber-preserved group（Ⅰ）,（12.9±1.81）d in medium-term-preserved group（Ⅱ）and（16.1±2.57）d in cryopreserved group（Ⅲ）. The MST in the cryopreserved group was evidently prolonged, showing a significant correlation compared with other two groups（P〈0.01）. The sections with HE staining revealed that the severity of inflammation in Ⅲ group was reduced compared with that of Ⅰ,Ⅱ group after 10d of keratoplasty. CONCLUSION： The postoperative rejection of penetrating keratoplasty in rats is decreased and rejection time is delayed in cryopreserved cornea.