NF-κB与COX-2的正相互作用诱导子宫颈癌细胞生长及抗凋亡

The positive interaction between NF-KB and COX-2 pathway induces cell growth and antiapoptosis in cervical cancer cells

目的探讨NF-κB和COX-2通路之间的相互作用对人子宫颈癌细胞株(Hela细胞)的生长及细胞凋亡的影响。方法应用细胞计数盒(CCK-8)检测细胞存活率;Hoechst 33258核染色检测凋亡细胞的形态及数量的改变;Western blot法检测Caspase-3、NF-κB和COX-2蛋白的表达。结果应用NF-κB抑制剂(PDTC)或COX-2抑制剂(NS-398)处理Hela细胞36 h能明显地抑制细胞存活率,PDTC或NS-398处理Hela细胞24 h能明显地促进Caspase-3表达,并增加凋亡细胞数量。PDTC处理Hela细胞能显著地抑制COX-2表达,另方面,NS-398处理Hela细胞能抑制NF-KB的表达。结论NF-κB和COX-2通路之间的正相互作用诱导Hela细胞生长及抑制细胞凋亡。

Objective To explore the influence of interaction between nuclear factor-κB （NF-κB） and cyclooxygenase-2 （COX-2） pathway on cell growth and apoptosis in Hela cells （human cervical cancer cell line）. Methods Cell viability was tested by cell counter kit （CCK-8） ; The changes in morphology and amount of apoptotic cells were detected by Hoechst 33258 nuclear staining; The expression levels of Caspase-3, NF-κB and COX-2 proteins were measured by Western blot assay. Results Exposure of Hela cells to PDTC （an inhibitor of NF-κB） or NS-398 （an inhibitor of COX-2） for 36 h markedly attenuated cell viability. Treatment of the cells with PDTC or NS-398 for 24 h enhanced the expression levels of Caspase-3, and increased amount of apoptotic cells, respectively. Treatment of Hela cells with PDTC significantly inhibited COX-2 expression. On the other hand, treatment of the cells with NS-398 reduced NF-κB expression. Conclusion The positive interaction between NF-κB and COX-2 pathway induces cell growth and inhibits apoptosis in Hela cells.