乌贼肝粉和鱼浆蛋白饲料对中华鳖抗氧化能力的影响

Effects of Squid Liver Meal and Soluble Fish Protein Concentrates Feeds on Antioxidant Capacity of Pelodiscus sinensis

本实验研究了饲料中添加乌贼肝粉和鱼浆蛋白对中华鳖抗氧化能力的影响。设计了3个实验组,对照组(白鱼粉组)、乌贼肝粉组和鱼浆蛋白组。3种实验饲料的营养成分相近,主要区别是乌贼肝粉组和鱼浆蛋白组的饲料中分别用乌贼肝粉和鱼浆蛋白取代了对照组饲料中5%的白鱼粉。用3种饲料分别养殖中华鳖(Pelodiscus sinensis)稚鳖8周,测定了稚鳖血清和肝组织的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、总抗氧化能力(T-AOC)以及血清碱性磷酸酶(AKP)和溶菌酶(LZM)的活性。结果发现,乌贼肝粉组稚鳖血清和肝MDA含量显著低于对照组,T-AOC高于对照组;鱼浆蛋白组稚鳖血清和肝MDA含量、血清T-AOC与对照组没有显著差异,但肝T-AOC显著低于对照组。实验结果表明,饲料中添加5%的乌贼肝粉能提高稚鳖的抗氧化能力。

Fish meal is the main protein source in the feed of Chinese soft-shelled turtle （Pelodiscus sinensis）. Squid liver meal and soluble fish protein concentrates have high protein content and many nutritional materials, and may be the alternatives to fish meal in turtle feed. Changes in t^ed composition can affect the antioxidant capacity of animals. Effects of supplementation of squid liver meal and soluble fish protein concentrates in feed on the antioxidant capacity of P. sinensis were studied in this experiment, with an attempt to explore which would be more suitable to replace fish meal in turtle ~ed. Three experimental groups were designed： control group （white fish meal group, WFM） , squid liver meal （SLM） group and soluble fish protein concentrates （SFP） group. Nutritional compositions of three diets were similar. For the diets of SLM and SFP, 5% white fishmeal of control diet was replaced with squid liver meal or soluble fish protein concentrates. The culture experiment lasted for 8 weeks. Activity of superoxide dismutase （SOD） , malondialdehyde （MDA） content and total antioxidant capacity （T-AOC） of liver and serum of turtle and aetivities of serum alkaline phosphatase andlysozyme were determined. The experimental data were analyzed with one-way ANOVA and Duncan＇s test. MDAs of liver and serum of SLM were both significantly lower than those of control group, and T-AOC of liver and serum of SLM were both higher than those of control group. No significant difference was showed in MDA of liver and serum and T-AOC of serum between SFP and control group, but liver T-AOC of SFP was significantly lower than that of control group. The results show that 5% supplementation of squid liver meal in diet can improve the turtle＇s antioxidant capacity, and supplementation of squid liver meal in diet is better than soluble fish protein concentrates for Chinese turtle.