一次力竭性离心运动损伤模型大鼠骨骼肌波形蛋白的表达

Cytoskeletal vimentin protein expression in rats with exhaustive eccentric exercise injury

背景:由于不同学者采用的实验方法不同,对离心运动后细胞骨架蛋白的变化仍有争议。目的:构建一次力竭性离心运动损伤大鼠模型,观察不同时刻骨骼肌细胞骨架波形蛋白表达的变化。方法:雄性48只SD大鼠建立下坡跑运动损伤模型,按运动时间分为安静对照组、运动后即刻组、运动后12 h组、运动后24 h组、运动后48 h组和运动后72 h组,每组8只。各运动组大鼠以速度16 m/min,坡度-16°进行跑台运动,运动100 min后,休息5 min,然后再运动100 min;安静对照组不做运动。应用抗波形蛋白抗体对大鼠骨骼肌波形蛋白进行免疫组化染色,通过观察其目标面积百分比的变化反映在一次力竭性离心运动后不同时刻大鼠骨骼肌细胞骨架波形蛋白的表达水平。结果与结论:大鼠骨骼肌细胞骨架波形蛋白目标面积百分比结果显示,安静对照组和运动后即刻组两组间差异无显著性意义(P>0.05);与运动后即刻组相比,运动后12 h组目标面积百分比略有增加,但差异无显著性意义(P>0.05);与运动后12 h组相比,运动后24 h组目标面积百分比略有增加,但差异无显著性意义(P>0.05);与安静对照组和运动后即刻组相比,运动后24 h组目标面积百分比有所增加(P<0.05);与运动后即刻组和运动后12 h组相比,运动后48 h组目标面积百分比明显增加(P<0.01);与运动后48 h组相比,运动后72 h组目标面积百分比有所下降(P<0.05),但没有恢复到安静时水平。提示一次力竭性离心运动后,大鼠骨骼肌波形蛋白出现不同程度的表达,在运动后12 h逐渐增加,运动后48 h达峰值,随后波形蛋白表达开始减少。

BACKGROUND：Due to different experimental methods, the change of cytoskeleton proteins after centrifugal movement is stil controversial. OBJECTIVE：To establish exhaustive eccentric exercise injury model in rats and to observe cytoskeletal vimentin protein expression at different time. METHODS：A total of 48 male Sprague-Dawley rats were randomly and equal y divided into six groups：quiet control group, immediately after exercise group, and 12, 24, 48, 72 hours after exercise groups. In the exercise groups, the rats were subject treadmil exercise at the speed of 16 m/min in a-16＆#176; slope, for 100 minutes at the interval of 5 minutes. The quiet control group maintained unchanged, without exercise. The cytoskeletal vimentin was detected with immunohistochemical staining using anti-vimentin antibody, and vimentin expression changes were observed at different time after exhaustive eccentric exercise through the changes of target area percentage. RESULTS AND CONCLUSION：The results of target area percentage showed that, there was no significant difference between quiet control group and immediately after exercise group （P〉0.05）. Compared with the immediately after exercise group, the target area percentage was slightly increased in 12 hours after exercise group, without significant difference （P〉0.05）. Compared with the 12 hours after exercise group, the target area＆amp;nbsp;percentage was also slightly increased in 24 hours after exercise group, without significant difference （P〉0.05）. Compared with quiet control group and immediately after exercise group, the target area percentage was increased in 24 hours exercise group （P〈0.05）. Compared with immediately and 12 hours after exercise groups, the target area percentage was increased significantly in 48 hours after exercise group （P〈0.01）. Compared with 48 hours after exercise group, the target area percentage began to decline in 72 hours after exercise group （P〈0.05）, which was higher than quiet control level. After exhaustive eccentric exercise, cytoskeletal vimentin begin to express at varying degrees. The vimentin expression is increased gradual y at 12 hours and reached the peak at 48 hours, then begins to decrease.