MCT2在福尔马林致痛模型大鼠大脑皮质中的表达变化

Changes of MCT2 expression in cerebral cortex after formalin-induced rat pain model

目的观察疼痛应激时,大脑第一躯体感觉皮质后肢区(primary somatosensory cortex hindlimb region,S1HL)神经元单羧酸转运蛋2(monocarboxylate transporters 2,MCT2)的表达变化,以探讨MCT2参与疼痛调制的机制。方法应用免疫组织化学(IHC)、Western blot和计算机图像分析法检测福尔马林致痛大鼠模型大脑S1HL内MCT2的表达变化。结果与正常组相比,模型鼠S1HL内MCT2阳性神经元的数量及IOD在1 h时增加,3 d时达高峰,到7 d时有所下降,但仍然高于正常水平(P<0.05)。Western blot结果显示,MCT2蛋白表达变化与MCT2阳性神经元的数量和IOD变化趋势一致。结论在疼痛应激状态下,大脑S1HL神经元MCT2的表达增强,提示MCT2参与了疼痛的产生、传递和调制过程。

Objective To investigate the changeofmonocarboxylate transporters 2（ MCT2） expression in cerebral cortex primary somatosensory cortex hindlimb region（S1HL）under the condition of pain stimulation, so as to explore the involvement of MCT2 in the pain adjustment mechanism in a rat pain model. Methods Immunohistochemical staining（IHC）, Western blot and Computing- image analysis system were used to detect the changes of MCT2 expression in the cerebral cortex S1HL of the formalin-induced rat pain model. Results Compared with the control group, the number and IOD of MCT2-positive neurons in the cerebral cortex S1HL of formalin-treated rats began to increase at 1h,reaching the highest level on 3d and declined on 7d ,but still higher than those in the control group （P〈0.05）.The Western blot showed that the alteration tendency of MCT2 expression was consistent with those of the number and IOD of MCT2-positive neurons. Conclusion Our results indicated that MCT2 is up-regulated under the condition of pain stimulation, which might be involved in the formalin-induced pain and behavior regulation.