脱细胞异种骨对星形胶质细胞的影响

Effects of xenogeneic acellular bone on the astrocyte cells

目的探讨脱细胞异种骨对星形胶质细胞增殖、迁移及细胞特性的影响。方法采用环氧交联技术制备脱细胞异种骨,将不同浸提浓度(0.05、0.1、0.2 g/mL)的异种骨浸提液加入含星形胶质细胞的培养基中。扫描电镜观察细胞形态;MTT法检测细胞增殖情况,确定最佳浸提浓度;Transwell小室检测细胞迁移情况;流式细胞仪检测细胞周期。结果扫描电镜示星形胶质细胞生长状态良好,呈星状结构;不同浸提浓度的异种骨浸提液中,0.05 g/mL的浸提液对细胞增殖有促进作用,0.05 g/mL为最佳浸提浓度;经该浸提浓度异种骨浸提液处理的细胞,迁移数量明显增多(P<0.05),但细胞各周期比例无明显变化(P>0.05)。结论经脱细胞脱基质处理的异种骨,对神经胶质细胞无明显毒性,对星形胶质细胞具有良好的细胞相容性。

Objective To observe the effects of xenogeneic acellular bone on the proliferation , migration and characteristic of astrocyte cells .Methods The xenogeneic bone was prepared by epoxy crosslinking technology after chemical and physical processing .Different concentrations （0.05, 0.1, 0.2 g/mL） of xenogeneic bone extracted liquid were added into the culture mediums with astrocyte cells .Scanning electron microscope （ SEM） was used for morphologic observation of astrocyte cells .MTT method was used detect the proliferation of the astrocyte cells , and to optimize the con-centration of xenogeneic bone extracted liquid .Transwell method and flow cytometry were used to access the cell migration and cell cycle , respectively .Results The astrocyte cells were polygonal , and were in a good state under SEM .The opti-mized concentration of xenogeneic bone extracted liquid was 0.05 g/mL, which had a positive effect on cell proliferation . In this concentration , the number of transwell cells was increased （ P〈0.05） , with no alteration on the cell cycle （ P〉0.05）.Conclusion Xenogeneic acellular bone has low influence on astrocyte cells , providing good biosecurity to astro-cyte cells.