壳聚糖即型水凝胶的理化性质、止血功能和生物相容性研究

Evaluation of a chitosan-based in situ forming hydrogel

报道了壳聚糖即型水凝胶,通过红外光谱和扫描电镜对该水凝胶进行了结构分析与表征,测定了其基本理化性质,并探讨了其止血功能和生物安全性。该水凝胶的红外光谱证实了西弗碱反应的发生,证明该水凝胶是由羟丙基壳聚糖和改性海藻酸钠交联而成;SEM照片显示该水凝胶的骨架是连续均一的蜂窝状网络结构;该水凝胶成胶迅速,最短成胶时间为10s,凝胶强度约为50g/cm2,溶胀率约为14.7%,pH值为7.5。在大鼠肝脏损伤出血模型的止血实验中,壳聚糖水凝胶具有优异的止血功能,止血时间和出血量均优于空白对照组(P<0.01),与纤维蛋白胶组无显著性差异(P>0.05)。壳聚糖水凝胶对细胞生长无明显抑制作用,其在体内降解伴随着组织炎症反应,炎症反应程度随材料的降解而减轻。

In situ forming hydrogels are important biomaterials in medical applications.A new class of in situ forming hydrogel derived from water-soluble chitosan and sodium alginate derivatives.The hydrogel was characterized by FT-IR spectroscopy.The surface morphology,gelation time,gel strength,swelling ratio and the pH value of the hydrogel were examined.As a potential hemostasis material,chitosan-based in situ forming hydrogel had splendid blood-control appearanced in the hemostasis experiment.The studies indicated that this hydrogel had no cytotoxicity and good histocompatibility.All the evidences provided a potential opportunity to use chitosan-based in situ forming hydrogel as a biodegradable and biocompatible hemostasis material.