检测鸭坦布苏病毒乳胶凝集试验的建立及初步应用

Establishment of a Latex Agglutination Test for Detection of Duck Tembusu Virus

以制备的鸭坦布苏病毒单克隆抗体致敏乳胶,建立检测鸭坦布苏病毒抗原的乳胶凝集方法。经优化后确定其最佳致敏抗体量为300μg、最佳致敏时间为3h、致敏温度为37℃。以乳胶凝集试验和RT-PCR对73份自然感染病例进行检测,分别检出阳性27、31份,两者检出的阳性符合率为87.1%。本方法快速、简便,适用于基层应用。

A latex agglutinin test for detection of duck Tembusu virus has been developed by sensitizing latex with monoclonal antibody of duck Tembusu virus WR strain .Under the optimum sensitization of latex particles with 300μg monoclonal antibody at 37℃ for 3 h , the latex agglutination test could reach a detection limit of 10-2.5 ELD50 duck Tembusu virus .A total of 73 samples collected from field cases were detected by latex agglutination test （27 positive） and RT-PCR （31 positive） ,indicating the coincidence rate of the two methods was 87.1% in practical utilization .The results showed that the latex agglutinin test for detection of duck Tembusu virus was facile ,rapid and visual ,which could be available for clinical utilization .