宫颈癌发生与SCARA5基因表达调控的关系及临床意义

Association of Regulation of SCARA5 Expression with Cervical Cancer Development and Its Clinical Significance

目的在前期研究中，发现官颈鳞癌组织中清道夫受体A类5型（scavenger receptor class Amember 5 putative，SCARA5）基因表达下调。本研究利用宫颈癌及癌前病变组织标本对SCARA5基因的mRNA和蛋白质表达水平进行定量分析．探讨宫颈癌发生与该基因表达调控的关系及临床意义。方法收集维吾尔族妇女宫颈鳞癌（cervical squamous cell carcinoma，CSCC）、官颈内上皮瘤变（cervical intraepithelial neoplasia．CINⅡ—Ⅲ）及宫颈炎患者的新鲜组织标本72例及石蜡包埋组织切片82例，采用定量RT-PCR和免疫组织化学方法，对SCARA5基因转录（mRNA）和蛋白质表达水平进行鉴定。结果随着宫颈炎发展到CIN和CSCC，SCARA5基因的转录（mRNA）表达水平梯度性下调，CSCC与CIN或官颈炎之间差异显著（P〈0．05），但是CIN与宫颈炎的差异无统计学意义。宫颈炎组织内SCARA5蛋白表达呈强阳性，在CINⅡ-Ⅲ中的缺失率增加，在宫颈癌阶段，其部分或完全缺失率最高，与宫颈炎存在显著差异（P〈0．05）。结论宫颈癌发生与SCARA5转录表达下调或蛋白质表达缺失存在密切关系，可能成为肿瘤诊断和预后的分子标志物。

Objective Previous studies have shown that the expression of scavenger receptor class A, member 5 （putative） （ SCARA5 ） was downegulated in cervical squamous cell carcinoma （CSCC） . The aim of this study was to investigate the association of regulation of SCARA5 expression with cervical carcinogenesis by quantitatively analyzing the mRNA and protein expression level of this gene in cervical carcinoma and its precursor lesions. Methods A total of 72 fresh tissue samples and 82 paraffin-embedded tissue slides were collected from Uyghur women with CSCC, cervical intraepithelial neoplasia （CIN）Ⅱ-Ⅲ or cervicitis, or healthy Uyghur individuals. The mRNA and protein expression of SCARA5 gene was determined by quantitative RT-PCR and immunohistochemistry, respectively. Result The mRNA expression level of SCARA5 was gradually down-regulated in cervicitis, CIN and CSCC in order, which reached statistically significant difference between CSCC and CIN or cervicitis （P 〈 0. 05） . But no difference was found in the mRNA expression of SCARA5 between CIN and cervicitis. Cervicitis tissues were strong-positive for SCARA5 protein, loss of SCARA5 expression increased in CIN Ⅱ-Ⅲ, and the rate of partial or total loss of SCARA5 expression was highest in CSCC, which was significantly different from that in cervicitis （P 〈 0. 05 ） . Conclusion The down-regulation of SCARA5 transcription or loss of protein expression is closely associated with cervical cancer development, and SCARA5 may become a molecular marker for the diagnosis and prognosis of CSCC.