胎盘儿茶酚氧位甲基转移酶rs4818、rs4633位点基因多态性与子痫前期的关系

Association between Polymorphisms of Placental Catechol-o-methyltransferase Gene rs4818、rs4633 and Preeclampsia

目的探讨胎盘儿茶酚氧位甲基转移酶(catechol-o-methyltransferase,COMT)基因rs4818、rs4633位点单核苷酸多态性(single nucleotide polymorphism,SNP)与子痫前期发病的关系。方法便利抽样法选择2011年3月至2012年8月在南方医科大学附属深圳妇幼保健院产科住院分娩的孕妇217例,其中子痫前期孕妇92例为子痫前期组,健康孕妇125例为对照组。收集两组孕妇产后胎盘组织,提取胎盘DNA,应用基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization time-of-flight mass spectrometer,MALDI-TOF-MS)平台及MassARRAY-IPLEX技术对COMT基因rs4818及rs4633位点进行检测,分析其基因型及等位基因分布情况。结果胎盘组织COMT基因rs4818位点基因型GG、GC、CC的基因型频率和G、C等位基因频率在子痫前期组与对照组之间的分布差异均无统计学意义(P>0.05)。两组胎盘COMT基因rs4633位点CC、CT、TT三种基因型频率和C、T等位基因频率在子痫前期组与对照组之间的分布差异无统计学意义(P>0.05)。结论胎盘COMT rs4818、rs4633单核苷酸多态性与子痫前期发生无关。

Objective To investigate whether polymorphisms of placental COMT gene are associated with preeclampsia,and to explore the role of COMT in the pathogenesis of preeclampsia.Methods By convenience sampling,217 patients were randomly selected including 92 patients with preeclampsia and 125 normal pregnant women.Placental tissue were collected and to extract DNA.Placental COMT（ rs4818 and rs4633） polymorphisms were genotyped by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry（ MALDI-TOF-MS） and MassARRAY-IPLEX.Results There were no significant differences in placental COMT rs4818 GG,GC,CC genotypes frequencies and G,C allele frequencies and between patients with preeclampsia and control group（ P〈0.05）.There were no significant difference between two groups of the placenta COMT rs4818locus CC,CT,TT in patients with preeclampsia and control group（ P〈0.05）.Conclusion Placental COMT rs4818 and rs4633 genotypes have no association with preeclampsia.