基因转染CLD1对大肠癌细胞株SW480粘附作用的影响

The adhesive effects of colorectal cancer cells SW480 by CLD1 gene transfection

目的探讨基因转染CLD1对大肠癌细胞株SW480粘附作用的影响。方法对SW480CLD1(转染CLD1的SW480细胞株)、SW480control(转染了pEGFP-C1的SW480细胞株)、SW480细胞株分别进行细胞同质粘附实验和细胞异质粘附实验。结果细胞同质粘附实验结果显示SW480CLD1细胞粘附细胞数量少于SW480control(P=0.000)和SW480细胞(P=0.000),SW480control的粘附细胞数与SW480无差异(P=0.376),粘附细胞数顺序为SW480CLD1<SW480control和SW480。异质粘附实验的结果显示SW480CLD1粘附细胞数多于SW480control(P=0.000)和SW480(P=0.000),差异有统计学意义。SW480control和SW480粘附细胞数无显著差异(P=0.334)。粘附细胞数顺序为SW480CLD1>SW480control和SW480。结论 CLD1基因转染后可减少SW480细胞之间的同质粘附作用,增加SW480细胞与Ⅳ型胶原的粘附性。

Objective To investigate the adhesive effects of colorectal cancer cells SW 480 by CLD1 gene transfection.Methods Cell homotypic adhesive tests and cell-matrix adhesive tests were performed on SW480CLD1 （SW480 by CLD1 gene transfection）,SW480control（SW480 by pEGFP-C1 transfection） and SW480 cells.Results The result of homotypic adhesive tests showed that the number of homotypic adhesive SW 480 CLD1 cells were less than those of SW480 control （ P =0.000 ） and SW480 cells （ P =0.000 ） .No significant difference was detected between SW480control and SW480 cells（P=0.376）.The sequence of number of adhesive cell were SW480CLD1 ＆lt;SW480control and SW480.The result of cell-matrix adhesive tests showed that the number of SW 480CLD1 adhesive cells were more than those of SW480control（P=0.000） and SW480 cells（P=0.000）, significant difference was detected.No signifi-cant difference was detected between SW480control and SW480 cells（P=0.334）.The sequence of number of adhesive cell were SW480CLD1 ＆gt;SW480control and SW480.Conclusion The homotypic adhesion was decrease between SW 480 cells by CLD1 gene transfection.The adhesion was increase between SW480 cells and collagen Ⅳmediated by CLD1 gene transfection .