高效液相色谱法快速测定植物油中苯并（а）芘残留量

采用C18（250 mm×4.6mm×5μm）反相色谱柱，在流动相为乙腈：水（88∶12，v/v），流速为1．0 ml/min，激发波长384 nm，发射波长406 nm，柱温35℃，进样量10μl 的条件下，建立高效液相色谱法快速测定植物油中苯并芘残留量的方法。结果：本方法的检出限为0.5μg/kg，加标回收率95%～103%，相对标准偏差RSD≤2.7%。该法具有样品预处理简便，试剂消耗少，分析时间短、精密度高等优点，适用于植物油中苯并芘残留量的高效测定。

A method for determination of benzopyrene in vegetable oil by High Performance Liquid Chromatography was established. Using C18 （250mm×4.6mm×5μm） reversed-phase column, with acetonitrile and H2O （88∶12,v/v） as mobile phase,flow rate was 1.0 ml/min, and excitation wavelength was 384nm and emission wavelength was 406 nm, and column temperature was 35℃, and injection volume was 10μl. Results：The detection limit was 0.5μg/kg, and the recovery rate arange from 95%to 103%, and relative standard deviation RSD was≤2.7%. This method is simple preparation, reagent consumption, short analysis time, precision advantages for vegetable oils and efficient determination of benzopyrene residues.