摘要
本试验旨在研究天冬氨酸(ASP)对脂多糖(LPS)刺激断奶仔猪肠道结构和黏膜抗氧化能力的影响。选取(21±1)d日龄断奶、平均体重(7.37±0.04)kg的杜×长×大三元杂交仔猪24头,随机分为对照组(基础日粮)、LPS组(基础日粮+LPS)、0.5%ASP组(基础日粮+0.5%ASP+LPS)、1.0%ASP组(基础日粮+1.0%ASP+LPS)4组,试验期24 d。试验第24天,分别给3个试验组的猪注射100μg/kg体重的LPS,对照组注射等量的生理盐水。注射后24 h屠宰,取小肠样品待测。结果表明:0.5%或1.0%ASP可显著缓解LPS刺激导致的空肠绒毛高度降低、隐窝深度升高和绒毛高度/隐窝深度的降低,增加回肠的绒毛高度、绒毛高度/隐窝深度(P<0.05);0.5%ASP对空肠超氧化物歧化酶、回肠谷胱甘肽过氧化物酶活性有提高的趋势(P<0.10);0.5%或1.0%ASP显著降低了空肠和回肠丙二醛含量(P<0.05)。以上研究结果显示,ASP有提高仔猪肠黏膜抗氧化相关酶的酶活趋势,降低过氧化物的产生,从而改善肠道形态结构。
The study was conducted to investigate the effect of aspartate (ASP) on antioxidant capacity of intestinal mucosa of weaned piglets challenged with lipopolysaccharide (LPS).Twenty-four weaned piglets (Duroc (Landrace (Large) weighing (7.37±0.04) kg were randomly assigned into 4 groups:control (basal diet); LPS (basal diet+LPS);0.5% ASP (basal diet+0.5% ASP+LPS); 1.0% ASP (basal diet+1.0% ASP+LPS).On day 24,the piglets in the LPS group,0.5% ASP group and 1.0% ASP group were injected intraperitoneally with 100 μg/kg BW LPS,and the piglets in the control group were injected with the same dose of physiological saline.After 24 h saline or LPS injection,all pigs were sacrificed to obtain intestinal mucosa for analysis.The results showed that 0.5% or 1.0% ASP alleviated the decrease of jejunal villus height and villus height/crypt depth and the increase of crypt depth induced by LPS,and increased ileal villus height and villus height/crypt depth (P<0.05).0.5% ASP had a tendency to improve the activities of superoxide dismutase in jejunum and glutathione peroxidise in ileum (P<0.10); 0.5% or 1.0% ASP significantly reduced the content of malondialdehyde in jejunum and ileum (P<0.05).In conclusion,ASP has tended to improve the activities of antioxidant enzymes,and alleviates histological intestinal injury induced by LPS challenge in piglets.
出处
《中国畜牧杂志》
CAS
北大核心
2014年第11期32-36,共5页
Chinese Journal of Animal Science
基金
教育部新世纪优秀人才支持计划(NCET-10-0158)
国家自然科学基金面上项目(31172222
31372318)
湖北省自然科学基金重点项目(2010CDA050)
湖北省国际科技合作项目(2011BFA008)
关键词
天冬氨酸
脂多糖
肠黏膜
抗氧化
断奶仔猪
aspartate
lipopolysaccharide
intestinal mucosa
antioxidant
weaned piglet