丁桂儿脐贴联合用药对小鼠止泻镇痛作用研究

Antidiarrheal and analgesic effects of Dingguier paste on the mouse models

目的：观察丁桂儿脐贴联合用药及丁桂儿口服用药对腹泻模型小鼠的止泻、镇痛作用。方法采用番泻叶小鼠腹泻模型、蓖麻油小鼠腹泻模型，按体质量随机分为模型组（10只）、丁桂儿赋性剂组（10只）、丁桂儿脐贴小（10只）、中（11只）、大剂量联合阳性药（11只）、阳性药组（11只）及丁桂儿口服组（11只），观察丁桂儿脐贴联合用药及丁桂儿口服后对腹泻模型的止泻作用。采用热板法、醋酸法，按正常对照组、丁桂儿脐贴小、中、大剂量联合阳性药、阳性药组及丁桂儿口服组给药，观察丁桂儿脐贴联合用药、丁桂儿口服后的镇痛作用。结果①丁桂儿脐贴小、中、大剂量＋地芬诺酯组、丁桂儿脐贴小剂量＋补脾益肠丸组、地芬诺酯组、补脾益肠丸组、丁桂儿口服组均可减少番泻叶小鼠腹泻模型2 h内稀便粒数[分别为（0.50±1.27）个、（2.27±2.90）个、（1.18±1.83）个，（2.00±2.26）个、（0.73±1.56）个、（1.91±2.95）个、（2.55±2.11）个]；丁桂儿脐贴小、大剂量＋地芬诺酯组可显著减少番泻叶小鼠腹泻模型4h内稀便粒数[分别为（6.70±2.11）个、（6.27±3.20）个]；丁桂儿脐贴大剂量＋地芬诺酯组可显著减少番泻叶小鼠腹泻模型6h内稀便粒数[（6.91±2.77）个]。②丁桂儿脐贴小剂量＋地芬诺酯组可减少蓖麻油小鼠腹泻模型2 h、4 h、6 h内稀便粒数[分别为（1.10±1.29）个、（3.20±0.63）个、（4.60±2.07）个]，丁桂儿脐贴中剂量＋地芬诺酯组可减少蓖麻油小鼠腹泻模型6 h稀便粒数[（4.64±2.45）个]，丁桂儿口服组可显著减少蓖麻油小鼠腹泻模型2 h、4 h、6 h内稀便粒数[分别为（0.45±0.82）个、（2.91±2.07）个、（4.27±2.15）个]。③丁桂儿脐贴小、中、大剂量＋罗通定组可提高小鼠给药后30 min、60 min、90 min、120 min痛阈[小剂量依次为（52.32±17.29）s、（56.24±12.48）s、（57.28±6.61）s、（58.58±4.70）s，中剂量依次为（48.27±19.35）s、（54.92±12.59）s、（55.91±11.48）s、（55.15±12.49）s，大剂量依次为（49.15±15.69）s、（56.24±11.89）s、（59.08±2.93）s]。④丁桂儿脐贴中剂量＋吲哚美辛组可明显延长乙酸刺激引起的疼痛潜伏期（14.72±5.99）min，丁桂儿脐贴小、中、大剂量＋吲哚美辛各组可显著减少乙酸刺激引起的小鼠扭体次数[分别为（7.62±8.31）次、（3.62±6.14）次、（11.25±9.46）次]。结论丁桂儿口服、丁桂儿脐贴联合用药有止泻、镇痛作用。

Objective To observe the antidiarrheal and analgesic effects of Dingguier paste on the mouse. Methods Tho diarrhea mouse models were made by senna leaf or castor oil to detect the antidiarrheal effect of Dingguier paste combination with other drugs. The hot plate test and acetic acid test were used to observe the analgesic effect of Dingguier oral medicine and Dingguier paste in combined use with other drugs. Results Compared with senna leaf model group, Dingguier paste low-dose, middle-dose, high-dose combined with diphenoxylate, Bupi-Yichang pill and Dingguier oral medicine group could decrease the number of feces in 2 h [Number of each group feces was（0.50±1.27）, （2.27±2.90）, （1.18±1.83）;（2.00±2.26）, （3.40±2.17）,＆amp;nbsp;（3.82±1.72）;（0.73±1.56）, （1.91±2.95）, （2.55±2.11）]. Dingguier paste low-dose and high-dose combined with diphenoxylate could decrease the number of feces in 4h [Number of each group feces was （6.70±2.11）, （6.27±3.20）]. Dingguier oral group decrease the number of feces in 6h [Number of feces was （6.91±2.77）]. Compared with castor oil model group, Dingguier paste low-dose combined with diphenoxylate could obviously decrease the number of feces in 2 h, 4 h and 6 h [Number of each group feces was（0.45±0.82）, （0.45±0.82）, （4.27±2.15）]. Dingguier paste middle-dose combined with diphenoxylate could decrease the number of feces in 6 h [Number of feces was （4.64±2.45）]. Dingguier oral group decrease the number of feces in 2 h, 4 h, 6 h [Number of each group feces was （0.45±0.82）, （2.91±2.07）, （4.27±2.15）].Compared with normal group, the threshold of pain stimulated by hot plate of the Dingguier paste each dose combined with rotundin could increased remarkable after 30 min, 60 min, 90 min and 120 min[The time of Dingguier paste low-dose was（52.32 ± 17.29）s, （56.24 ± 12.48）s, （57.28 ± 6.61）s, （58.58 ± 4.70）s; the time of Dingguier paste middle-dose was （48.27±19.35）s, （54.92±12.59）s, （55.91±11.48）s, （55.15±12.49）s;the time of Dingguier paste high-dose was （49.15±15.69）s, （56.24±11.89）s, 60 s is（59.08±2.93）s]. Compared with normal group, the eclipse time of pain irritated by acetic acid of the Dingguier paste middle-dose combined with indomethacin could increased remarkable（14.72±5.99）min, the writhing frequency could decrease of the Dingguier paste each dose combined with indomethacin[Number of each dose writhing frequency was （7.62±8.31）, （3.62± 6.14）, （11.25±9.46）]. Conclusion Dingguier paste in the combination use with other drugs and Dingguier oral medicine may have some antidiarrheal and analgesia effects.