葡萄糖激酶调节蛋白基因rs780094多态性与儿童青少年血脂关系的研究

Association between rs780094 polymorphism in GCKR and plasma lipid levels in children and adolescents

目的：探讨葡萄糖激酶调节蛋白（GCKR）基因rs780094的多态性与儿童青少年血脂水平的关系。方法选取1026名7～18岁中小学生为研究对象。由专职人员记录学生一般情况和既往病史，检测身高、体重，并采集清晨空腹肘静脉血，测定血清TC、TG、HDL-C和LDL-C水平。利用基质支持的激光释放/电离飞行时间质谱分析（MALDI-TOF MS）进行GCKR基因rs780094位点的基因型检测。采用多元线性回归和多元logistic回归分析基因多态性与血脂水平的关系。结果调整年龄、年龄的平方和性别，GCKR基因rs780094多态性A等位基因与TC、TG和LDL-C的水平增加存在相关性（b＝0.06 mmol/L，P＝0.037；b＝0.09 mmol/L，P＜0.001；b＝0.05 mmol/L，P＝0.040）；rs780094多态性与TG、LDL-C异常也存在相关性（OR＝1.60，95%CI：1.30～1.97，P＜0.001；OR＝1.35，95%CI：1.02～1.80，P＝0.036）。结论 GCKR基因rs780094位点的多态性与儿童青少年血脂水平有关，A等位基因可能是血脂增高的遗传因素。

Objective To investigate the association between rs780094 polymorphism in glucokinase regulatory protein （GCKR） and plasma lipid levels in children and adolescents. Methods 1 026 Chinese children aged 7 to 18 years were recruited,with anthropometric measurements,detection of plasma lipid levels and genotyping of rs780094 performed. Relationships between polymorphism and plasma lipid levels were tested,using multivariate linear regression and logistic regression. Results A-allele of rs780094 in GCKR was associated with increased TC,TG and LDL-C levels（b=0.06 mmol/L,P=0.037;b=0.09 mmol/L,P〈0.001;b=0.05 mmol/L,P=0.040） under the additive model adjusted for age,age square and gender. The rs780094 in GCKR was also associated with abnormal levels of TG and LDL-C（OR=1.60,95%CI：1.30-1.97,P〈0.001;OR=1.35,95%CI：1.02-1.80,P=0.036）. Conclusion The rs780094 in GCKR was associated with plasma lipid levels in children and adolescents while A-allele of rs780094 might serve as genetic factor for the increased plasma lipid levels.