基质金属蛋白酶-2和血管内皮生长因子在喉癌中的表达及意义

Expressions of MMP-2 and VEGF in laryngeal carcinoma and their clinicopathology meanings

目的探讨基质金属蛋白酶-2(MMP-2)和血管内皮生长因子(VEGF)在喉癌中的表达情况及临床意义。方法在全自动图像分析仪下采用免疫组化方法分别检测经临床及病理证实的54例喉癌、23例声带息肉和15例喉部正常黏膜组织的MMP-2、VEGF表达情况。结果喉癌组织中MMP-2的表达率为72.22%(39/54),与声带息肉(47.83%)和喉部正常黏膜组织(40.00%)比较,差异有统计学意义(P<0.05)。VEGF在喉癌中的表达率为70.37%(38/54),与声带息肉(39.13%)和喉部正常黏膜组织(46.67%)比较,差异有统计学意义(P<0.05)。MMP-2、VEGF的表达与喉癌的临床分期、淋巴结转移及浸润深度均有一定相关性。结论 MMP-2和VEGF可能共同参与喉癌的浸润和转移,与肿瘤的侵袭性生长有一定的关系。

Objective To study the expressions of matrix metalloproteinase-2（MMP-2）and vascular endothelial growth factor （VEGF）in laryngeal carcinoma and their clinicopathological significance.Methods The immuno-histochemical technique was used to detect the expression staining results of MMP-2 and VEGF in 54 cases of laryngeal carcinoma,23 cases of polyps of vocal cord and 1 5 cases of normal laryngeal mucous tissues verified by clinicopathology under the automatic image analyzer.Results The ex-pression rates of MMP-2 and VEGF were 72.22%（39/54）and 70.37%（38/54）in the laryngeal carcinoma tissue,which were sig-nificantly higher than 47.83% and 39.13% in polyps of vocal cord and 40.00% and 46.67% in the normal laryngeal tissues respec-tively,the differences between them had statistical significance（P〈0.05）.The expressions of MMP-2 and VEGF had certain corre-lation with the clinical stage,lymph node metastasis and.infiltration depth of laryngeal carcinoma.Conclusion MMP-2 and VEGF may jointly participate in the process of infiltration and metastasis in laryngeal and have certain relation with the tumorous invasive growth.