大肠癌组织中MMP-2与TIMP-2的表达及临床意义

Expression and clinical significance of MMP-2 and TIMP-2 in human colorectal carcinoma

目的研究基质金属蛋白酶-2(MMP-2)与组织金属蛋白酶抑制剂-2(TIMP-2)在大肠癌中的表达,探讨二者在大肠癌发展过程中的意义。方法应用免疫组化法检测52例大肠癌、20例大肠腺瘤和20例正常大肠黏膜组织中MMP-2与TIMP-2的表达,并分析二者表达与性别、年龄、肿瘤发生部位、浸润程度、组织分化程度及淋巴结转移等临床指标间的关系。结果MMP-2在大肠正常黏膜、大肠腺瘤和大肠癌中的表达强度依次增加,而TIMP-2在三者中的表达强度则依次降低。MMP-2在浸润程度深的大肠癌中的表达高于浸润程度浅的,低分化的高于中高分化的,有淋巴结转移的高于无淋巴结转移的;TIMP-2的表达则在浸润程度深的低于浸润程度浅的,低分化的低于中高分化的,在有淋巴结转移的低于无淋巴结转移的。MMP-2与TIMP-2在大肠癌组织中的表达与患者的性别、年龄及肿瘤发生部位无显著相关性,MMP-2与TIMP-2在大肠癌中的表达呈负相关。结论 MMP-2与TIMP-2表达的失平衡与大肠癌的浸润和转移密切相关。

Objective To study the expression of matrix metalloproteinases-2（MMP-2）and tissue inhibitor of metalloproteinas-es-2（TIMP-2）in colorectal carcinoma and to investigate its significance in the development process of colorectal cancer.Methods To detect the expression of MMP-2 and TIMP-2 in 52 cases of colorectal cancer,20 cases of colorectal adenoma and 20 cases of colorectal normal mucosa tissue by the immunohistochemical method.To analyze the relationship between their expression with the clinical indexes of sex,age,tumor location,tissue differentiation degree,infiltration and lymph node metastasis.Results The inten-sity of MMP-2 expression in the colorectal normal mucosa,adenoma and colorectal carcinoma in turn was increased,while the ex-pression intensity of TIMP-2 in the three tissues in turn was reduced.The MMP-2 expression in deep infiltration was higher than that in shallow infiltration,low differentiation was higher than high differentiation and lymphatic metastasis was higher than non-lymphatic metastasis;while the TIMP-2 expression in deep infiltration was lower than that in shallow infiltration,low differentiation was lower than high differentiation and lymphatic metastasis was lower than non-lymphatic metastasis.The expression of MMP-2 and TIMP-2 in colorectal carcinoma had no obvious differences in patient′s sex,age and tumor location.MMP-2 was negtively corre-lated with TIMP-2 in colorectal carcinoma.Conclusion The imbalance expression of MMP-2 and TIMP-2 is closely correlated with the infiltration and metastasis of colorectal carcinoma.