磁场对结球甘蓝种子萌发的影响及机理研究

Effect of Magnetic Field on Brassica oleracea L. var. capitata L. Seed Germination and Studies on Its Mechanism

以结球甘蓝种子为试材,采用正交试验和均匀试验方法,研究了磁场强度、作用时间及处理前种子浸泡时间对各发芽指标的影响以及各影响因素的主次顺序,并对磁场参数中磁场强度和作用时间进行大范围筛选,更进一步从可溶性蛋白含量、丙二醛含量、抗氧化酶活性等方面探讨磁场处理提高种子活力的作用机理。结果表明：适宜的磁场处理可以促进种子萌发,提高种子活力,以20℃蒸馏水浸泡8 h后1 000-3 500 GS磁场处理1-6 min为宜,但长时间、大强度磁场处理反而抑制种子萌发;磁场强度为种子萌发的主要影响因子,作用时间和浸泡时间的主次顺序因发芽指标的不同而不同;与对照相比,磁场处理的种子萌发期间可溶性蛋白含量降低或升高的速率、MDA含量下降的幅度和速率、3种抗氧化酶活性上升的速率均加快,从而提高种子活力。

Taking Brassica oleracea L. var. capitata L. seed as experimental material,the effect of magnetic field intensity,and magnetic acting time and seed soaking time before treatment on seed germination index and primary and secondary relations between all factors were studied by orthogonal test and uniform test method.Then the large scale screening of magnetic field intensity and magnetic acting time were carried out to further investigate the mechanism of using magnetic field treatment to improve seed vigor from the view points of soluble protein content,malondialdehyde content,and antioxidant enzyme activity.The results indicated that suitable magnetic field treatment could promote seed germination and improve seed vigor.Magnetic field intensity was the main factor affecting seed germination.The primary and secondary order of action time and soaking time were varied with different germination indexes. Seed germination was inhibited by too high magnetic field intensity for a long time. Soaking seed in 20 ℃ distilled water for 8 hours,1-6 minutes was appropriate treatment by 1 000-3 500 GS magnetic field.After magnetic field treatment,the soluble sugar and protein contents were increased,MDA content decreased,and enzyme activities of antioxidant system were increased,thus the seed germination effect was improved.