大理某医院检验报告单带菌情况分析

Investigation on the Bacterial Contamination of Laboratory Sheets in a Hospital of Dali

目的:研究检验报告单的带菌情况,并行菌属及菌种类型分析。方法:在检验报告单上直接取样增菌接种于血琼脂平板和麦康凯平板培养,用形态学检验及生化反应鉴定到种。结果:1 000份检验报告单,血琼脂平板共生长细菌719株,其中葡萄球菌属518株占72.04%,肠道杆菌175株占24.34%,枯草杆菌26株占3.62%。麦康凯平板共生长细菌369株,其中葡萄球菌属299株占81.03%,肠道杆菌70株占18.97%。结论:检验报告单主要带菌为葡萄球菌和肠道杆菌,其中葡萄球菌以表皮葡萄球菌和金黄色葡萄球菌为主,肠道杆菌以耶氏菌和志贺氏菌为主。

Objective：To investigate the species of bacterial contamination of laboratory sheets. Methods：laboratory sheets were direct sampled and enrichment culture then inoculating blood Columbia agar plates and MacConkey Agar plates. Isolated bacteria were identified by morphology,Gram stain and biochemical test. Results：From 1 000 laboratory sheets,after inoculate,there were 719 bacteria growing in blood agar platein. The differential strains grew on blood Columbia agar plates and their infection rates were： Staphylococcus,518 strains,infection rates was 72.04%;Enteric bacilli,175 strains,infection rates was 24. 34%;Bacillus subtilis,26 strains,infection rates was 3.62%. The differential strains grew on MacConkey Agar plates and their infection rates were ： Staphylococcus,229 strains,infection rates was 81.03%;Enteric bacilli,70 strains,infection rates was 18.97%. Conclusion：In the inspection report,the main bacteria are mainly including Staphylococcus and Enterobacteriaceae. Staphylococcus is mainly Staphylococcus epidermidis or Staphylococcus aureus,and Enterobacteriaceae is mainly Yersinia or Shigella.