人感染田鼠巴贝斯虫的实验室诊断一例

Laboratory diagnosis for one human case infected with babesia

目的通过回顾1例人感染巴贝斯虫的实验室资料,以积累经验,提高诊断水平。方法研究红细胞(RBC)、血小板(PLT)直方图,白细胞(WBC)VCS散点图;观察外周血、骨髓涂片中虫体的形态;从患者外周血中提取的DNA核酸用疟原虫、巴贝斯虫种、巴贝斯虫属特异性引物进行聚合酶链反应(PCR)。结果 RBC直方图波峰降低,宽度增加,PLT直方图无异常,WBC VCS散点图非WBC区有颗粒团;外周血、骨髓涂片见大量拟似恶性疟原虫虫体;PCR产物测序结果经BLAST比对,结果显示与巴贝斯虫和田鼠巴贝斯虫18s核糖体DNA序列分别有96%和99%的同源性。结论感染的虫体为田鼠巴贝斯虫。

Objective To review the laboratory data of one human case infected with babesia,in order to gain experience and improve the diagnosis level.Methods The histograms of red blood cell （RBC）,platelet （PLT）and white blood cell（WBC）VCS scatterplot were analyzed.The parasite morphology was observed in peripheral blood and bone marrow smears.DNA extracted from peripheral blood of the patients with plasmodium vivax,voles babesia and babesia DNA-specific primers by polymerase chain reaction （PCR）.Results RBC histogram peak reduced,and its width increased.PLT histogram had no abnormality.The non-WBC area of WBC VCS scatter plot had particle group. Peripheral blood and bone marrow smears emerged a large number of quasi plasmodium falciparum parasites.The results of PCR product sequencing showed that the sequencing results had 96%and 99%homology with the 18s ribosomal DNA sequence of voles babesia and babesia.Conclusions The patient is infected with voles babesia.