苦参碱对人前列腺癌PC-3M细胞周期与凋亡的影响

Effect of matrine on cell cycle and apoptosis of human prostate cancer PC-3M

目的研究苦参碱(MR)对人前列腺癌PC-3M细胞周期与凋亡的影响。方法稳定培养PC-3M细胞株系,经0(空白组)、30、60、120μM浓度MR给药72 h后,应用四甲基偶氮唑盐(MTT)法检测MR对PC-3M抑制率,碘化丙啶(PI)染色检测细胞形态学的变化,免疫组织化学法检测细胞周期蛋白D1(Cyclin D1)表达,Western blot法检测细胞外调节蛋白激酶1/2(ERK1/2)磷酸化蛋白水平。结果与空白组比较,MR给药后有效抑制PC-3M的细胞增殖(P<0.01),并促进癌细胞凋亡。同时,MR能显著减少Cyclin D1的表达以及下调内源性p-ERK1/2水平(P<0.01)。结论苦参碱可发挥有效的抗癌作用,推测其机制为抑制癌症细胞增殖并介导细胞凋亡,以及抑制内源性ERK1/2/Cyclin D1通路。

Objective To assess the effect of matrine on cell cycle and apoptosis of human prostate cancer PC-3M. Methods Stably cultured PC-3M cell lines was treated MR 0（blank group）,30,60,120 μM concentrations,respectively,for 72 h. MTT assay was employed for MR-mediated inhibition rate of PC-3M. Propidium iodide（PI） staining was used to observe morphological changes. Cyclin D1 expression was analyzed by immunohistochemistry,the phosphorylated level of extracellular regulated kinase1 /2 was tested via Western blot assay. Results Compared with the blank group,MR administration led to the effective anti-proliferation of PC-3M cells（P ＆lt; 0. 01）,and promoted apoptosis in tumour cells（P ＆lt; 0. 01）. Meanwhile,MR administration also distinctly reduced the Cyclin D1 expression and down-regulated endogenous p-ERK1 /2 level（ P ＆lt; 0. 01）. Conclusion Overall,matrine plays the pronounced anticancer effect,which the mechanism is linked to the suppression of cancer cell proliferation and mediation of apoptosis,as well as the inhibition of endogenous ERK1 /2 /Cyclin D1 pathway.