免疫增强剂对鸡球虫体液免疫指标的影响

Influence of Immunoenhancers on the Parameters of Humoral Immunity against Chicken Coccidiosis

艾维茵雏鸡于 7日龄和 14日龄时分别以 10 0 0、50 0 0个 E.Tenella卵囊 /只免疫两次 ,并于首免时注射卡介苗 (BCG)或黄芪多糖 (APS) 1次 ,2 1日龄以 5万和 2 5万卵囊攻击。试验鸡于 7、14、2 1日龄和 2 8日龄采血 ,EL ISA测定血清中 Ig G、Ig M抗体的含量。结果表明 :BCG具有促进球虫免疫鸡体液免疫应答的作用 ,而

The chickens were immunized with 1000 and 5000 oocysts of E.tenella at ages of 7 and 14 days respectively,and injected with BCG or APS at the first immunization,then challenged with 5000 or 25000 oocysts/chicken at 21 days old.Sera from experimental chickens were collected at 7,14,21,28 days after the test for detecting IgG and IgM antibodies by ELISA.It indicated that BCG could enhance humoral immunization ability,however,APS was not apparent.