鹿血清蛋白抗氧化肽体外抗氧化性及其机理研究

Antioxidant Properties and Possible Action Mode of Deer Blood Hydrolysate

在碱性蛋白酶最适条件下水解鹿血清蛋白,水解时间为0~5 h,鹿血清蛋白抗氧化肽的水解度（DH）随着水解时间增加而增加（P〈0.05）,鹿血清蛋白抗氧化肽还原能力、清除自由基的作用和Cu2＋螯合能力随着水解时间的增加而显著提高（P〈0.05）,鹿血清蛋白抗氧化肽能有效抑制硫代巴比妥酸反应物质（TBARS）生成（P〈0.05）。结果表明,鹿血清蛋白抗氧化肽的抗氧化活性取决水解时间,鹿血清蛋白抗氧化肽是有效的抗氧化剂。

Deer blood was hydrolyzed by Alcalase for 0 to 5 h, and the degree of hydrolysis （DH） of protein hydrolysates increased with increasing hydrolysis time （P〈0. 05）. The reducing power, radicals scavenging activities and Cu2＋-chelation ability of deer blood hydrolysate （DBH） were significantly enhanced with increasing hydrolysis time （P〈0. 05）. The antioxidant activity of DBH was inhibited by thiobarbiturie acid-reactive substance （TBARS） values in a liposome-oxidizing system （P 〈 0. 05）. The results indicated that antioxidant activity of DBH depended on hydrolysis time, and the hydrolyzed deer blood could be a potent food antioxidant.