1-磷酸鞘氨醇对角膜血管新生的影响

The effect of Sphingosine-1-phosphate on rat corneal neovascularization

目的评价1-磷酸鞘氨醇(S1P)对大鼠角膜新生血管(CNV)的影响。方法 20只SD大鼠随机分成对照组和实验组,制做角膜微囊袋并分别植入空白缓释微粒体和含S1P的微粒体,术后1d,7d,14d观察各组大鼠角膜新生血管的生长情况。结果对照组未见明显新生血管生长而实验组新生血管生长明显,差异有统计学意义(P<0.05),实验组CNV的面积、累积钟点数和血管长度随观察时间推移而增多(P<0.05)。结论 1-磷酸鞘氨醇可促进角膜的血管增生,作用机理可能与激活角膜缘附近血管内皮细胞S1PR通路有关。

Objective To explore the effect of Sphingosine-l-phosphate on rat corneal neovascularization. Methods Twenty Sprague-Dawley rats were divided into control group and experiment group. Pellets made of VEGF, poly-2-hydroxyleth- ylmethacry-late and sucralfate were implanted into the corneal stroma. Biomicroscopic features of corneal neovascular were observed on 1, 7, 14 day after the implantation. Results The corneal neovascularization of control group is more apparent than experiment group and the difference was statistically significant （ P 〈 0. 05 ）. The area, cumulative number of hours and length of blood vessels of corneal neovascularization in the experiment group increased greatly as time went by （P 〈 0. 05）. Conclusion Sphingosine-l-phosphate can promote the blood vessel hyperplasia and the mechanism of action is probably relevant to activation of the corneal limbal of vascular endothelial cell receptor.