hTERT基因转染对人神经元活力的影响

Influence of hTERT on human embryonic cerebral cortex neuron

目的:观察人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)对Aβ诱导的人胚胎大脑皮质神经元细胞活力的影响。方法:人胚胎大脑皮质神经元原代培养后,以hTERT重组腺病毒转染神经元,用Aβ25-35诱导神经元的凋亡,72 h后用MTT检测各组细胞数量和活力,并计算其保护率。结果:hTERT重组腺病毒转染后72 h进行Aβ干预,此时hTERT对神经元的保护力为29.8%,活细胞的数量及细胞活力(0.310±0.033)明显高于非转染组(0.237±0.081),P=0.002,差异有统计学意义。结论:Aβ干预后的神经元,其活力明显下降,而hTERT重组腺病毒转染可以促进神经元存活,对Aβ干预的人胚胎大脑皮质神经元具有保护作用。

Objective：To observe the effects of hTERT on human embryonic cerebral cortex neuron after Aβ invention. Methods：Primary cerebral cortex neurons of human embryo that was cultured were transfected with hTERT. After the neu-rons were dealt with Aβ25-35 ,the activity of neurons was measured by MTT. Result：After 72 hours transfection with pSU-CMV-hTERT,the neurons were intervented of Aβ,the neurons could resist the toxic effects of Aβ effeciently,the protec-tive index is about 29. 8%,and the activity of neurons（0. 310 ± 0. 033）was significanly higher than that without treat-ment（0. 237 ± 0. 081）detected by the MTT,P=0. 002. Conclusion：The vitality of the neurons intervented with Aβob-viously was decreased. however,the hTERT transfection could promote the survival of neurons,and show the protective effects to neurons.