摘要
目的观察胃癌组织中微小RNA(miRNA)-181a(miR-181a)与其靶基因Kruppel样因子6(Kruppel like factor 6,KLF6,又称锌指转录因子9或核心启动子元件结合蛋白)的表达变化,并探讨其意义。方法 9例胃癌患者,手术时留取癌组织及癌旁组织,抽提其中的总RNA及蛋白质,采用实时荧光定量PCR检测标本中的miR-181a,Western blot检测KLF6蛋白。结果胃癌组织中miR-181a的表达量(2-△△Ct)为(1.981±1.080),癌旁组织miR-181a的表达量为(0.394±0.093);癌组织KLF6蛋白灰度值为(0.241 6±0.135 5),癌旁组织为(0.540 2±0.05 5),两种组织中的miR-181a、KLF6蛋白表达量相比,均p﹤0.01;miR-181a与KLF6蛋白的表达呈负相关(r=-0.590,p﹤0.01)。结论胃癌组织中miR-181a表达明显上调,KLF6蛋白表达明显下降;结合前期实验研究,我们推测miR-181a可能通过转录后基因沉默机制使KLF6蛋白表达下降,从而促进胃癌的发生发展。
Objective To investigate the expression and significance of microRNA（ miRNA）-181a（ miR-181a） and its target gene Kruppel like factor 6（ KLF6） in gastric carcinoma tissues. Methods The gastric carcinoma and adjacent non- tumorous tissues were collected（ from 9 patients） respectively and the total RNA and protein were extracted routinely. The miR- 181a was detected by Real- time quantitative PCR. KLF6 protein were detected by Western blot. Results In gastric carcinoma tissues,the miR- 181a and KLF6 protein was（ 1. 981 ± 1. 080）,（ 0.241 6 ±0.135 5）,respectively,whereas in the adjacent non-tumorous tissues,the miR-181a and KLF6 protein was（ 0. 394 ± 0. 093）,（ 0. 540 2 ± 0. 055）,respectively. The differeace between the expression of miR- 181a and KLF6 protein in these two tissues was statistically significant（ all p ﹤ 0. 01）. There was a remarkable inverse correlation between miR- 181a and KLF6 protein levels was observed（ r =- 0. 590,p ﹤ 0. 01）. Conclusion The expression of miR- 181a was significantly up- regulated in gastric carcinoma tissues,whereas the KLF6 protein was markedly decreased. Based on the previous research,we speculate that miR- 181a may inhibit KLF6 expression by post- transcriptional gene silencing to restrain gastric carcinoma occurrence and progress.
出处
《现代医院》
2014年第5期9-12,共4页
Modern Hospitals
基金
广东省自然科学基金(编号:NO.10151006001000016)
