柑桔果实浮皮发生机理及控制途径研究Ⅱ.特早熟温州蜜柑果实浮皮与衰老的关系

Mechanism of Puffiness and the Methods of Control in Citrus Fruit Ⅱ.Relation between Puffiness and Senescence of the Early maturing Satsuma Mandarin

为探明柑桔果实浮皮与衰老的关系 ,以特早熟温州蜜柑品种胁山为试材 ,采前对其幼果和枝叶喷施赤霉素、亚精胺、醋酸钙 ,测定果皮及果肉的多聚半乳糖醛酸酶 (PG)、超氧化物歧化酶 (SOD)和丙二醛 (MDA )等生理生化指标 .结果表明 :胁山品种随着果实浮皮的出现 ,PG活性提高 ,果皮的细胞膜脂质过氧化作用产物 MDA逐渐积累 ,而果皮和果肉的 SOD活性则急剧下降 .胁山果实浮皮的根本原因是膜脂过氧化作用引起的果皮早衰 .采前喷施赤霉素、亚精胺、醋酸钙对 MDA含量 ,PG,SOD活性均有一定调节作用 。

Early maturing citrus variety was used in this study to investigate the relation between puffiness and senescence.Polygalacturonase (PG),malondialdehyde (MDA),superoxide dismutase (SOD) in peel,sarcocarp were determined by spraying GA 3, Spd and 1% (CH 3COO) 2Ca on the young fruits,twigs and leaves before fruit picking.The results show that with the development of fruit puffiness, MDA accumulates in peel rapidly, the PG activities in peel increase quickly,but, SOD activities of peel decrease acutely. Therefore, the reason of puffiness was the premature senescence of peel caused by cell membrane lipid peroxidation. GA 3,Spd and(CH 3COO) 2Ca can regulate the MDA contents and the SOD activities while GA 3 had the most significant effect.