利用RAPD分子标记对30份山茶属种质资源进行分类分析

Classification and Analysis of 30 Camellia Germplasm Resources by Using RAPD Molecular Markers

[目的]对30份山茶属种质资源进行分类分析.[方法]利用RAPD分子标记技术研究30份山茶属种质资源的遗传多样性,并进行分类分析.[结果]17个RAPD引物共扩增出138条带,多态性条带为129条,多态性比率为93.5％,材料间的遗传相似系数（GS）变化范围为0.605 ～ 0.855.[结论]利用NTSYS软件对RAPD扩增结果进行聚类分析,可将30份资源分为5大类群,其分类结果与张宏达分类体系基本一致.

[Objective] To classify and analyze 30 Camellia germplasm resources.[Method] Using RAPD molecular markers,the genetic diversity of 30 Camellia germplasm resources was studied and classified.[Result] The result showed that 138 bands were amplified by 17 RAPD primers and 129 polymorphic bands （93.5％）.The variance range of genetic similarity （GS） among 30 accessions of Camellia germplasm was 0.605-0.855.[Conclusion] The dendrogram was constructed by using the NTSYS software based on RAPD data indicated that 30 Camellia germplasm were divided into 5 classes,and the classification results were basically the same as the Zhang Hongda classification system.