产表面活性剂菌的筛选和验证实验研究

Experimental Study on Surfactant Bacteria Screening and Validation

[目的]探究废弃钻井液中产表面活性剂菌株的性能。[方法]以废弃的钻井液为原材料,利用生物表面活性剂的性质进行产表面活性剂菌株的培养和筛选,并且将筛选出来的产表面活性剂菌进行排油活性和乳化性能的测定。[结果]筛选出的产表面活性剂菌排油圈的直径约为4 cm,乳化层初始较厚,1 d后变薄。[结论]筛选出的产表面活性剂菌排油活性较好,但乳化性能不稳定。

[Objective] The research aimed to explore the performance of surfactant producing bacteria of waste drilling fluid.[Method] Taking waste mud as raw material,surfactant producing strain was trained and selected using properties of bio-surfactants,and oil discharge activity and emulsifying properties of surfactant producing bacteria produced and screened out were determined.[Result] Oil ring of surface active agent screened was about 4 cm,the initial emulsion layer was thick,and it was becoming thinning a day later.[Conclusion] Oil extraction activity of surface active agent was good,but the emulsification performance was instable.