鸡新城疫山东强毒株的分离鉴定及致病性研究

Isolation,Identification and Pathogenicity of Newcastle Disease Virus Strains in Chickens of Shandong Province

从山东省不同地区的发病鸡群中分离了 6株病毒 ,经HA、HI试验、病毒回归试验和血清中和接种鸡胚试验确定所分离的毒株为新城疫病毒。分别对 6个毒株的MDT、ICPI和IVPI进行了测定 ,结果表明这些毒株为鸡新城疫强毒 ,但各个毒株的致病性有所差异。

Six virus strains were isolated from affected chickens in some areas of Shandong province,and characterized as Newcastle disease virus by hemagglutination/hemagglutination_inhibition test,challenge with 6_week specific pathogen free(SPF) chickens and inoculation of embryos after neutralization using particular sera.Mean death time(MDT)of embryos,intracerebral pathogenicity index in 1_day_old chicks(ICP)and intravenous pathogenicity index in 6_week_old chickens(IVPI)were determined respectively for isolates.The results showed that the six strains were highly vilulent and produced different pathogenicity in chickens and embryos.