大豆种质对菌核病的耐病性评价及资源筛选

Evaluation and Identification of Tolerance to Sclerotinia Sclerotiorum in Soybean Cultivars

[目的]探明大豆种质资源对大豆菌核病的耐受性(部分抗性)水平,挖掘耐病种质资源是选育抗病品种的前提和基础。[方法]通过茎中可溶性色素法和离体叶柄接种法对130份大豆品种(系)进行抗/耐病性评价。[结果]2种方法的评价结果相关系数达到0.69,通过色素法鉴定得到耐病材料6份,中度耐病材料16份,通过叶柄接种法获得高抗材料13份,囊括了色素法筛选得到的全部耐病材料和部分中耐材料。[结论]说明色素法作为一种间接鉴定方法,筛选标准较叶柄接种法更为严格和精准,适合大量种质资源的耐病性筛选。

[Objective] To explore the tolerance or partial resistance to Sclerotinia Sclerotiorum in soybean germplasms in appropriale methods and determine the basis of breeding for disease resistance.[Methods] Two methods were used,including soluble pigment in stem and petiole inoculation for resistance/tolerance identification were used to evaluate 130 soybean cultivars or lines.[Results] The correlation coefficient of results from two methods was 0.69.A total of 6 accessions were found to be tolerance and 16 were showed medium tolerance to Sclerotinia Sclerotiorum through measuring soluble pigment.in stem after treated with oxalic acid.A total of 13 resistant accessions were identified to be resistant to Sclerotinia Sclerotiorum by petiole inoculation method,including all the 6 tolerant and partial medium tolerant accessions that were screened by the method of soluble pigment in stem.[Conclusion] The soluble pigment in stem method was stricter than petiole inoculation method in resistance identification and can be used for identification of tolerant germplasms to Sclerotinia Sclerotiorum from plenty of soybean accessions.