利用动态光散射研究包覆材质对纳米茄红素胶囊粒径的影响

Study on Effect of Coating Materials on Particle Size of Lycopene Nanocapsules by Dynamic Light Scattering

利用微乳化技术与复合团聚法制备了单层、双层及多层包覆的纳米茄红素胶囊,并以动态光散射法先后测量分析了包覆材质对这些纳米胶囊的粒径大小及其分布的影响。研究结果表明,以碱处理动物胶单层包覆的纳米茄红素胶囊,其在pH为6.5的磷酸盐缓冲液中会出现比较严重的聚集现象;以碱处理动物胶与k-红藻胶双层包覆的纳米茄红素胶囊,其在pH为5.5的磷酸盐缓冲液中也会出聚集现象,但比单层包覆时要小很多;而多层包覆的纳米茄红素胶囊可在pH为5.5的磷酸盐缓冲液中具有良好的分散性,且没有出现聚集现象。

Single-layer, double-layer and multi-layer coating lycopene nanocapsules are prepared by the micro-emulsion technology and composite reunion method, and the effect of coating materials on particle size and its distribution is analyzed by dynamic light scattering method. The studied results show that the more serious aggregation can be produced in phosphate buffer of pH=6.5 for the single-layer lycopene nanocapsules coated by alkali-treated gelatin. Compared with the single-layer coating lycopene nanocapsules, the weaker aggregation can be observed in phosphate buffer of pH=5.5 for the double-layer lycopene nanocapsules coated by alkali-treated gelatin and x-carrageenan. However, the multi-layer coating lycopene nanocapsules can be dispersed in stability in phosphate buffer of pH=5.5 and there is no aggregation.