杜仲叶的化学成分研究

Chemical Constituents of the Leaves of Eucommia ulmoides

通过硅胶、Sephadex LH-20和高效液相半制备色谱反复柱层析、纯化,从杜仲叶中分离得到14个化合物,利用理化性质和波谱分析分别鉴定为山萘酚(1)、槲皮素(2)、陆地锦苷(3)、紫云英苷(4)、(+)-1-hydroxylpinoresinol(5)、(+)-1-hydroxy-6-epipinoresinol(6)、pinoresinol-4-O-glucoside(7)、thunberginol C(8)、(+)-medioresinol 4'-O-β-D-glucopyranoside(9)、7-hydroxycoumarin(10)、绿原酸甲酯(11)、十八碳二烯酸(12)、丁二酸(13)、β-谷甾醇(14)。化合物6、8、10、12和13为首次从该属植物中分离得到。

Fourteen compounds were isolated from the leaves of Eucommia ulmoides Oily. by several methods, including silica gel, reversed phase RP-18 column chromatographies ,Sephadex LH-20, and HPLC. Their structures were identified by NMR and EI-MS spectra, as kaempferol （ 1 ）, quercetin （2）, hirsutin （3）, astragalin （4）, （ ＋ ） -1-hydroxylpinores- inol （5）, （ ＋ ） -1-hydroxy-6-epipinoresinol （ 6 ）, pinoresinol-4-O-glucoside （ 7 ）, thunberginol C （ 8 ）, （ ＋ ） -mediores- inol 4- O-β-D-glucopyranoside （ 9 ） ,7-hydroxycoumarin （ 10 ）, methylch]orogenate （ U ）, leinoleic acid （ 12 ）, butanedlo- ie acid （13） ,β-sitosterol （14）. Compounds 6,8,10,12 and 13 were isolated from E. ulmoides for the first time.