柴胡属植物HPLC指纹图谱及聚类分析

HPLC Fingerprintingand Cluster Analysis of Bupleurum Plants

为给柴胡属植物的合理使用和基源选育提供理论依据,本文用HPLC法建立了北柴胡标准指纹图谱,确立了14个色谱峰为共有峰,测定了13批不同基源柴胡的指纹图谱及相似度,并对包括北柴胡在内的23批柴胡进行聚类分析。实验结果显示13批不同基源柴胡相似度均高于0.8,与北柴胡具有较高的相似性,可作为优质柴胡资源进一步研究。聚类分析结果显示竹叶柴胡聚为一类,北柴胡聚为一类,马尔康柴胡、马尾柴胡和抱茎柴胡聚为一类,与竹叶柴胡基源更为接近,聚类分析结果与传统分类结果具有一致性,可用于柴胡属植物的基源鉴定。

In order to provide scientific evidence on rational use and origin select of Buplqurum plants,the HPLC stand- ard fingerprint of Bupleurumchinense was established and 14 common peaks were confirmed. 13 batches of Bupleurum samples were analyzed by similarity evaluation. In addition, cluster analysis was performed with 23 batches of Bupleurum samples including B. chinense. The results suggested that thedegree of similarity were over 0.8, and all of them showed highly similarity to B. chinense. It shown that B. marginatum can be further studied as a superiority resource of Bupleu- rum. Classification of different Bupleurum sampleswas obtained using systematic cluster method. The results indicated that B. marginatumwas clustered as one class;B, chinensewas clustered as one class;B, malconense, B. microcephalum and B. longicaule var. amplexicaule were clustered as one class. This result was consistent with the traditional classification and can be used for the identification of origins of Bupleurum samples.