SPE-HPLC法分离合欢皮中抗血管新生的活性组分

Isolation of Components with Anti-angiogenesis Activity from Albizia julibrissin by Solid Phase Extraction Coupled with Preparative HPLC

应用固相萃取-高效液相色谱法(SPE-HPLC),从合欢皮乙醇提取物-正丁醇相中分离得到若干个组分,再用HMEC-1细胞活性检测以及HPLC分析鉴定,筛选得到具有较高抑制新生血管活性的组分II-4(IC50=1.45±0.11μg/mL),且组成较简单。本法简单快速,可以为中药材有效组分的快速分离提供新的方法,也为后续进一步分离得到细胞活性有效单体化合物提供组成比较简单的合欢皮活性组分提取物。

In this study, several components with anti-angiogenesis activity were obtained from the n-butanol fraction of ethanol extract of Albiziajulibrissin by solid phase extraction （SPE） coupled with preparative HPLC. The anti-angiogenesis effect and the composition analysis of the isolated components were monitored on HMEC-1 cells and by HTLC method, respectively. Finally, the component II-4 with higher anti-angiogenesis activity on HMEC-1 cells was obtained （ IC50= 1.45 ±0.11μg/mL） ,and its composition was identified. The SPE-HPLC method was simple and fast,and can he used for rapid separation of Chinese herbal medicine at the same time it provided a reliable experimental materials for further separation of monomer compounds.