基于咪唑盐离子液体固定GO_x于Au/石墨烯复合材料电极表面的葡萄糖酶传感器研究

Study of a glucose enzyme sensor based on[C_(10)-mim^+]Br^-fixing GO_x on Au /graphene nanocomposites

利用长链离子液体特殊的性质,用其固定葡萄糖氧化酶(GOx)于Au/石墨烯电极表面组装成Nafion/GOx/[C10-mim+]Br-/Au/Gr修饰电极,然后用其测定葡萄糖。用透射电镜表征氧化石墨烯和Au/氧化石墨烯的形貌发现,金纳米颗粒很均匀的分散在石墨烯表面,并不存在团聚现象。电化学数据显示,Nafion/GOx/[C10-mim+]Br-/Au/Gr修饰电极对葡萄糖具有很好的催化作用,葡萄糖浓度在6.0×10-5~2×10-3mol/L范围内呈良好的线性关系(R=0.997),检出限为1.6×10-5mol/L。

In this paper, a novel nano -composite material modified electrode （Nafion/GOx/[ C l0-mim^＋ ] Br-/ Au/Gr） was prepared by utilizing long chain ionic liquids （an immediate conversion from a viscous liquid to a stable, homogeneous gel when a certain amount of water is added into them） immobilizing GOx on an Au/ graphene electrode surface. The morphologies of the graphene oxide and Au/graphene oxide nanocomposite film were investigated by transmission electron microscopy, and the Au nanoparticles are uniformly dispersed on the graphene oxide surface and non - existent aggregates. Electrochemical response of glucose on the Nafion/GOx/ [C10-mim^＋] Br-/Au/Gr modified electrode was investigated. The research results showed that the modified electrode exhibited excellent electrocatalytic effect. Under the optimal experimental conditions, the reduction peak current on the Nation/ GOx/[ C10-mim^＋] Br^-/Au/Gr modified electrode was proportional to the concentration of glucose in the range from 6.0 × 10^-5 to 2 ×10^-3 mol/L with a correlation coefficient of 0. 997. The detection limit was 1.6×10^-5 mol/L. The sensor has good stability and selectivity. The sensor provides new means for the determination of biomolecules.