黔岭淫羊藿总黄酮类成分对去势雌性大鼠骨质疏松症的实验研究

Effect of total flavones of Epimedium leptorrhizum on osteoporosis in ovariectomized rats

目的:观察黔岭淫羊藿总黄酮类成分(YYH-C)对去卵巢大鼠骨质疏松的防治作用。方法:将去卵巢大鼠随机分为模型组、YYH-C低、中、高剂量组(生药0.7,1.4,2.8 g·kg-1)和阳性药补佳乐组(0.15 mg·kg-1),另设假手术组。连续灌胃给药3个月,测定血清中甲状旁腺激素(PTH)、I型前胶原N末端前肽(PINP)、碱性磷酸酶(ALP)、钙(Ca)和磷(P)含量;取左侧股骨和腰椎,进行骨计量学指标测定,包括骨密度、骨钙磷含量和骨灰重/干重百分比;取右侧股骨进行骨组织形态学观察。结果:和假手术组比较,模型组大鼠PTH,ALP明显升高,PINP,血钙水平明显降低。YYH-C 3个剂量组均能明显对抗PINP的降低,低、中剂量组尚能明显抑制PTH的升高,高剂量组能明显增加血钙含量,但对ALP的增高无明显抑制作用,各组之间血磷含量无明显差异;模型组动物股骨、椎骨骨密度、灰重/干重比值和Ca,P含量与假手术组比较均明显降低;YYH-C高剂量组能明显提高骨密度,3个剂量组均能明显增加灰重/干重比值和股骨、椎骨Ca,P含量;YYH-C能显著升高股骨近端的骨小梁平均厚度、骨小梁面积、骨小梁面积百分比以及骨干的皮质骨面积百分比等,增加骨小梁表面成骨细胞数,减少破骨细胞数。结论:YYH-C能有效控制去卵巢所致骨质疏松模型大鼠的骨量丢失,防止骨显微结构的改变,抑制骨吸收亢进,进而对抗去卵巢后的高转换型骨质疏松症。

Objective： To observe the effect of total flavones of Epimedium leptorrhizum（YYH-C） on osteoporosis in ovariectomized rats.Method： Ovariectomized female rats were randomly divided into the model group,YYH-C lower,middle and high dose（0.7,1.4,2.8 g·kg- 1） groups,the positive drug Bujiale（0.15 mg·kg- 1） group,and the sham group.The rats were orally administrated with drugs for three months.Parathyroid hormone（PTH）,procollagen I N-terminal peptide（PINP）,alkaline phosphatase（ALP）,calcium（Ca） and phosphrous（P） in serum were detected.Femur bones and vertebrae bones of left side were collected to determined bone metrological indexes,including bone mineral density（BMD）,bone Ca,and bone ash weight /dry weight percentage.Femur bones of right side were collected to for a morphological observation of bone.Result： Compared with the sham group,the model group showed significantly higher PTH and ALP content but obviously lower PINP and Ca content.The three YYH-C 3 groups could resist the decrease of PINP.Specifically,low and middle dose groups could remarkably inhibit the increase of PTH,and the high dose group could increase the Ca content in serum,but without significant effect on the rise in ALP.There was no significant difference in P content in serum in each group.BMD,ash weight /dry weight percentage,Ca and P content of the model group were significantly lower than those in the sham group.The high dose YYH-C group could significantly increase BMD.All of the three YYH-C groups could notably increase ash weight /dry weight percentage and Ca,P content in femur bones and vertebrae bones.YYH-C could significantly increase average thickness,area,area percentage of bony trabeculae,cortical bone area percentage of femoral shaft and the number of osteoblasts on the surface of bony trabeculae,and decrease the number of osteoclasts.Conclusion： YYH-C can effectively control the bone mass loss of rats with ovariectomy-induced osteoporosis,prevent the changes in bone microstructure,and inhibit bone absorption,so as to resist high turn-over osteoporosis after ovariectomy.