合成法氏囊素和LCGF与卵黄抗体对鸡IBD的治疗作用

Treatment of chicken IBD with Synthetic bursin,LCGF and Yolk Antibody

应用溶血空斑试验和溶血分光光度测定法分析了合成法氏囊素 ( BS)及其结构类似物—肝细胞生长因子 ( L CGF)对机体抗体形成细胞的影响 ,并进一步研究了二者对IBD的治疗作用。结果证明 ,合成 BS和L CGF均能显著提高小鼠抗体形成细胞的增殖 ,促进小鼠抗 SRBC抗体分泌 ( P <0 .0 1 )。治疗试验表明 ,合成 BS和 LCGF或卵黄抗体单独应用 ,均可显著提高 IBD发病鸡群的成活率 ( P <0 .0 1 )。BS,LCGF单独应用或与卵黄抗体结合的治疗效果均显著高于试验剂量的卵黄抗体 ( P <0 .0 1 ) ,高剂量的 BS能促进病鸡法氏囊损伤的修复。

The effects of Bursin(BS) and its structural analog (liver cell growth factor, LCGF) , in vivo, on antibody secreting cells from mouse spleen were determined through Hemolytic Plaque Formation Tests and Spectrophotometric determination .Further , the therapeutic efficacy of the BS, LCGF and Yab(yolk antibody) to chicken IBD was investigated as well. The results testified that both BS and LCGF can enhance the number of PFCs and the amount of the anti SRBC antibody secreted by the spleen B cells in mouse (P<0.01). The therapy tests show that all the BS , LCGF and Yab can increase the survival rates of IBD chicken (P<0.01) and the rates of the chicken treated with BS , LCGF,BS+Yab and BS+Yab are higher than those treated with Yab alone(P<0.01).the BS at high dose can promote the rehabilitating of the Bursa of Fabricius damaged by IBDV.