中国野生葡萄抗白粉病基因的RAPD标记

RAPD marker of resistance gene to Uncinula necator in Chinese wild Vitis

利用随机扩增多态性 DNA ( RAPD)技术 ,对抗病×感病的葡萄种间杂交组合白河 - 35 - 1×佳利酿的亲本及其 F1 代的 2 4株杂种进行了抗白粉病的遗传分析 ,从 196个随机引物中获得了 1个与中国野生葡萄抗白粉病基因连锁的 RAPD标记 OPV0 3- 1380 ,并在中国野生葡萄华东株系和欧洲葡萄品种中进行了分析验证。

The genetic analysis of the resistance to powdery mildew of Chinese wild Vitis was carried out with the parents and 24 F 1 individuals of interspecific hybridization combination Baihe 35 1×Carignane (Resistant × Susceptible) by Randomly Amplified Polymorphic DNA (RAPD) technique.A total of 196 random primers were screened on the DNA of resistant and susceptible materials.OPV03 1380,a RAPD marker,which linked to powdery mildew resistant gene in Chinese wild Vitis ,was obtained.Additionally,the OPV03 1380 was tested among clones of V.pseudoreticulata of Chinese wild Vitis and varieties of V.vinifera .